摘要
目的研究水杨酸、酵母提取物、水解酪蛋白、高糖、高盐、超声等诱导子对甘草悬浮培养细胞中甘草酸积累的影响。方法向细胞悬浮液中加入各种诱导子或对细胞悬浮液进行超声处理。结果加入10 mg·L-1水杨酸可使甘草酸的含量为对照组的1.86倍。低浓度的酵母提取物对甘草酸的积累有显著的促进作用,加入1 mg·L-1酵母提取物时,甘草酸的含量为对照组的3.71倍,达到14.20μg·g-1。添加100 mg·L-1水解酪蛋白时,甘草酸的含量为对照组的1.69倍。高盐胁迫对甘草酸的积累有较高的影响,其含量为对照组的1.96倍。结论甘草细胞悬浮培养中,添加水杨酸、酵母提取物、水解酪蛋白、高糖、高盐、超声等诱导子是提高细胞中甘草酸含量的有效方法之一。
Abstract
OBJECTIVE To investigate the effects of different elicitors(salicylic acid,yeast extract,casein hydrolysate,high sucrose,high salt,ultrasonic stimulation) on glycyrrhizin production in Glycyrrhiza uralensis cells.METHODS The glycyrrhizin content in Glycyrrhiza uralensis cells was analyzed by HPLC after the treatment of elicitation.RESULTS 10 mg·L-1 Salicylic acid resulted in an increase of glycyrrhizin content by about 1.86 times.The highest glycyrrhizin content of 14.20 μg·g-1 was obtained by the addition of 1 mg·L-1 yeast extract,and it was 3.71 times as that of control.The use of 100 mg·L-1 casein hydrolysate on the 5th day of the culture resulted in an increase of glycyrrhizin content by 1.69 times.With the high salt treatment,the glycyrrhizin content was increased by 1.96 times.CONCLUSION The addition of the salicylic acid,yeast extract,casein hydrolysate,high sucrose,high salt to the medium and the ultrasonic stimulation was effective to enhance the glycyrrhizin content in Glycyrrhiza uralensis cells.
关键词
甘草 /
细胞悬浮培养 /
甘草酸含量 /
诱导子
{{custom_keyword}} /
Key words
Glycyrrhiza uralensis /
cell suspension culture /
glycyrrhizin content /
elicitors
{{custom_keyword}} /
卞爱华;高文远;王娟.
不同诱导子对甘草悬浮培养细胞中甘草酸积累的影响[J]. 中国药学杂志, 2008, 43(22): 1690-1693
IN i-hu;GO Wen-yun;WNG Jun.
Effect of Elicitors on Accumulation of Glycyrrhizin in the Suspension Culture Cells of Glycyrrhiza uralensis[J]. Chinese Pharmaceutical Journal, 2008, 43(22): 1690-1693
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] DING Q L，HUI L ，YAN D，et al.Biocatalytic properties of a novel crude glycyrrhizin hydrolase from the liver of the domestic duck[J] .J Mol Catal （B：Enzym），2006，43(1-4)：148-152.
[2] ARMANINI D，NACAMULLI D，FRANCINI-PESENTI F，et al. Glycyrrhetinic acid，the active principle of licorice，can reduce the thickness of subcutaneous thigh fat through topical application[J] . Steroids，2005，70(8)：538-542.
[3] CINATL J，MORGENSTERN B，BAUER G，et al.Glycyrrhizin，an active component of liquorice roots，and replication of SARS-associated coronavirus[J] .Lancet，2003，361(9374)：2045-2046.
[4] ZHANG W，FUMSAKI S. Production of anthocyanins by plant cell cultures[J] .Biotechnol Bioprocess Eng，1999，4(4)：231-252.
[5] CHEN W，YU Q L，GAO W Y，et al.The studies on the callus of licorice Glycyrrhiza uralensis Fisch[J] .China J Chin Mater Med (中国中药杂志)，2005，30(9)：712-715.
[6] WUA J Y，WONGA K，HO K P，et al. Enhancement of saponin production in Panax ginseng cell culture by osmotic stress and nutrient feeding[J] .Enzyme Microb Technol，2005，36(1)：133-138.
[7] TAMAKI E，MORISHITA I，NISHIDA K，et al.Verfahren zum herstellen eines lakritzenextraktartigen produktes als tabak-aromastoff：Germany，2146580[P] . 1972-05-31.
[8] KOBAYASHI M，NOGUCHI K，SANKAWA U. Formation of chalcones and isoflavones by callus culture of Glycyrrhiza uralensis with different production patterns [J] .Chem Pharm Bull，1985，33 (9)：3811-3816.
[9] HAYASHI H，FUKUI H，TABATA M. Examination of triterpe- noids produced by callus and cell suspension cultures of Glycyrrhiza glabra[J] .Plant Cell Rep，1988，7(7)：508-511.
[10] HAYASHI H，SAKAI T. Formation of soyasaponin in licorice cell suspension cultures[J] .Phytochemistry，1990，29(10)：3127-3129.
[11] KLESSING D F，MALAMY J. The salicy acid signal in plants[J] .Plant Mol Boil，1994，26(5)：1439-1458.
[12] XU L S，XUE X F，FU C X，et al.Efects of methyl jasmonate and salicylic acid on phenylethan0id glycosides synthesis in suspension cultures of Cistanche deserticola[J] .Chin J Biotechnol (生物工程学报)，2005，21(3)：402-405.
[13] ENDRESS R. Plant Cell Biotechnology[M] . Berlin：Springer-Verlag，1994：216-223.
[14] CHEN H，CHEN F. Effect of yeast elicitor on the secondary metabolism of Ti-transformed Salvia miltiorrhiza cell suspension cultures[J] .Plant Cell Rep，2000，19(7)：710-717.
[15] YANG S H，LIU X F，GUO D A. Efects of diferent additives on accumulation of flavonoids in Glycyrrhiza uralensis Callus[J] . Chin Pharm J (中国药学杂志)，2006，41(2)：96-99.
[16] LIN L D，WU J Y，HO W K，et al.Ultrosound-induced phys- iological effects and secondary metabolite (saponin) production in panax ginseng cell cultures[J] .Ultrasound Med Biol，2001，27(8)：1147-1152.
[17] WU J，LIN L. Elicitor-like effects of low-energy ultrasound on plant (Panax ginseng) cells：induction of plant defense responses and secondary metabolite production[J] .Appl Microbiol Biotechnol，2002，59(1)：51-57.
[18] LIU S，ZHONG J J. Effects of potassium ion on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax ginseng[J] .J Biotechnol，1996，52(2)：121-126.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家中医药管理局中医药科学技术研究专项项目(04-05ZP10);国家科技支撑计划项目(2006BAI06A16-02)
{{custom_fund}}
