OBJECTIVE To investigate the inhibition of rhynchophylline(Rhy),components of Uncaria rhyrchophylla(Miq.),on vascular smooth muscle cells(VSMCs) proliferation induced by angiotensin Ⅱ(AngⅡ),and explore the possible mechanisms.METHODS The effects of Rhy on VSMCs proliferation induced by Ang Ⅱ 10-6 mol·L-1 were determined by cytometry and methyl thiazolyl tetrazolium(MTT) assay,the effects of Rhy on the expressions of c-myc,constitutive NO synthase(NOS) and rat hypertension related gene-1(rHRG-1) mRNA were detected by RT-real time PCR,respectively.RESULTS Rhy(3×10-6～3×10-7 mol·L-1) depressed VSMCs proliferation induced by Ang Ⅱ in a concentration-dependent manner,and increased the contents of NO and the activities of NOS in cultured cell.Furthermore,Rhy up-regulating the expressions of NOS and rHRG-1 mRNA,and down-regulated the expression of c-myc mRNA,respectively.CONCLUSION Rhy obviously attenuated the cultured rat VSMCs proliferation induced by Ang II.The mechanisms are,at least partly,due to an increase of the NOS activity,and promoting the synthesis and release of NO,and up-regulating the expressions of NOS and rHRG-1 mRNA,and down-regulating the expression of c-myc mRNA.
LI Yn-ju;SUN n-sheng;YU Li-mei;WU Qin.
Inhibition of Rhynchophylline on Cultured Vascular Smooth Muscle Cells Proferation Induced by Angiotensin Ⅱ [J]. Chinese Pharmaceutical Journal, 2008, 43(21): 1621-1623
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