摘要
目的从新疆光果甘草中制备总黄酮(LGFs)及单体成分光甘草定(glabridin,GLB),并研究它们的抗氧化活性。方法用常规方法制备LGFs和GLB;利用HL-60细胞中的Myeloperoxidase/H2O2/HClO氧化系统和肝微粒体中的细胞色素P450/NADPH氧化系统做体外抗氧化实验模型,对LGFs和GLB的抗氧化活性进行测定。系统中自由基诱发程度由探针物DCFH-DA的氧化产物DCF的浓度来检测。银杏叶提取物EGb761用作阳性对照物。结果在两种体外模型中,LGFs和GLB均显示较强的抗氧化活性,它们清除自由基活性强度为EGb761≥GLB>LFs。结论甘草总黄酮及其中的主要活性成分GLB在HL-60细胞和肝微粒体氧化系统中具有很强的抗氧化活性。这些结果可部分解释甘草药理作用与其抗氧化预防活性的关系。
Abstract
OBJECTIVE To prepare licorice general flavonoids(LGFs) and its purified constituent glabridin from Xinjing Glycyrrhiza glabra L,and to investigate their antioxidant properties.METHODS The routine method was applied for obtaining LGFs and glabridin.Two different in vitro oxidative systems: the enzymatic oxidative system MPO(myeloperoxidase) /H2O2/HOCl in human HL-60 cell line and the cytochrome P450/NADPH-reductase within hepatic rat microsomes were used for testing their antioxidant properties.The free radical scavenger Ginkgo biloba extract(EGb 761) was used as reference.Cellular and microsomal reactive oxygen species(ROS) formation was determined by the fluorescence of 2′,7′-dichlorofluorescein(DCF) originated from 2′,7′-dichlorofluorescein diacetate(DCFHDA) probe oxidation.RESULTS In both models,the preparied substances exhibited globally remarkable antioxidant properties and were classified by their ROS inhibition capacity as follows: EGb761≥GLB>LFs.CONCLUSION The present study demonstrates that licorice general flavonoids and its active principle glabridin have relatively high antioxidant activities in both oxidative models.These results could partially explain the pharmacological actions of licorice with antioxidant prophylactic activities.
关键词
新疆光果甘草 /
总黄酮 /
光甘草定 /
HL-60细胞 /
肝脏微粒体 /
抗氧化活性
{{custom_keyword}} /
Key words
Xinjiang Glycyrrhiza glabra L /
licorice general flavonoids /
glabridin /
HL-60 cell line /
hepatic microsomes /
antioxidant activities
{{custom_keyword}} /
木合布力·阿布力孜;毛新民;热娜·卡斯木;马淑燕;季晓娟.
新疆光果甘草黄酮类成分的抗氧化活性[J]. 中国药学杂志, 2008, 43(21): 1617-1620
MO Xin-min;REN Ksimu;M Shu-yn;JI Xio-jun.
Antioxidant Activities of Flavonoids from Xinjiang Glycyrrhiza glabra[J]. Chinese Pharmaceutical Journal, 2008, 43(21): 1617-1620
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] FUHRMAN B，VOLKOVA N，KAPLAN M，et al.Antiatherosclerotic effects of licorice extract supplementation on hypercholesterolemic patients： increased resistance of LDL to atherogenic modifications，reduced plasma lipid levels，and decreased systolic blood pressure[J] . Nutrition，2002，18 (3)：268-273.
[2] KUNTZ S，WENZEL U，DANIEL H. Comparative analysis of the effects of flavonoids on proliferation，cytotoxicity，and apoptosis in human colon cancer cell lines[J] . Eur J Nutr，1999，38 (3) ：133-142.
[3] YU X Q，XUE C C，ZHOU Z W，et al. In vitro and in vivo neur oprotective effect and mechanisms of glabridin，a major active isoflavan from Glycyrrhiza glabra (licorice)[J] . Life Sci，2008，82 (1)：68-78.
[4] WANG Y S ，XU L，MA K，et al. Protective effects of Ginkgo biloba extract 761 against glutamate-induced neurotoxicity in cultured retinal neuron[J] . Chin Med J（中华医学杂志），2005，118 (11)：948-952.
[5] MA S Y，ABLISE M，KAHAER B，et al. Study on the preparation technology of isoflavon glabridin from Glycyrrhiza glabra L[J] . J Xinjiang Med Univ (新疆医科大学学报)，2007，30（7）：692-694.
[6] ABLISE M，LEININGER-MULLER B，DAL WONG C，et al. Synth esis and in vitro antioxidant activity of glycyrrhetinic acid derivatives tested with the Cytochrome P450/NADPH system[J] . Chem Pharm Bull，2004，52(12)：1436-1439.
[7] ABLISE M， MAO X M，KASIM R，et al . Antioxidant property of general cistanosides in human HL-60 cell line[J] . Chin Pharm Bull (中国药理学通报)，2008，24(3)：362-364.
[8] CHOI D W，LEININGER-MULLER B，YOUNG C K，et al. Diff- erential role of CYP2E1 binders and isoniasid on CYP2E1 protein modification in NADPH-dependent micrisomal oxidative reactions：Free radical scavenging activity of isoniasid[J] . Free Rad Res，2002，36(3)：893-903.
[9] LEBORGNE L，MAZIERE J C，MAZIERE C，et al. Oxidative stress，atherogenesis and cardiovascular risk factors[J] . Arch Mal Coeur Vaiss，2002，95(5)：805-814.
[10] PETROVSKY N，SILVA D，SCHATZ D A. Prospects for the preve ntion and reversal of type 1 diabetes mellitus[J] . Drugs，2002，62(6)：2617-2635.
[11] ARUOMA O I. Characterization of drugs as antioxidant prophylactics[J] . Free Radic Biol Med，1996，20(5)：694-705.
[12] RAJBHANDARI I，TAKAMATSU S，NAGLE D G. A new dehydrog eranylgeraniol antioxidant from Saururus cernuus that inhibits intracellular reactive oxygen species (ROS)-catalyzed oxidation within HL-60 Cells[J] . J Nat Prod，2001，64 (6)：693-695.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家十一五科技支撑项目基金资助(2006BAI06A18-20)
{{custom_fund}}
