摘要
目的测定中肺合剂中防己诺林碱、粉防己碱的含量。方法样品经预处理后采用RP-HPLC同时测定它们的含量。色谱条件:色谱柱为Agilent Zorbax Elipse XDB-C18;流动相为0.06%三乙胺-乙腈溶液(44∶56);流速为1.0 mL·min-1;检测波长设在240 nm;柱温30℃。结果防己诺林碱在0.437 5～17.5 mg·L-1与粉防己碱在0.875～35.0 mg·L-1内,峰面积与其浓度具有良好的线性关系。平均加样回收率分别为89.30%～94.35%(防己诺林碱),90.08%～95.74%(粉防己碱)。结论本方法简便、快速、准确,可同时测定中肺合剂中防己诺林碱和粉防己碱的含量。
Abstract
OBJECTIVE To establish a method for the determination of fangchinoline and tetraridrine in Zhongfei mixture.METHODS The separation was performed on an Agilent Zorbax Elipse XDB-C18 column at 30 ℃ and detected at 240 nm,the mobile phase consisted of 0.06% triethylamine and acetonitrile(44∶56) at a flow rate of 1.0 mL·min-1.RESULTS The calibration curves of fangchinoline and tetraridrine had excellent linearity between the range of 0.437 5-17.5 mg·L-1 and 0.875-35.0 mg·L-1.The average recoveries of spiked with sample were 89.30%-94.35% for fangchinoline and 90.08%-95.74% for tetraridrine.CONCLUSION The method is sensitive,convenient and accurate,and is suitable for the simultaneous determination of fangchinoline and tetraridrine in Zhongfei mixture.
关键词
中肺合剂 /
防己诺林碱 /
粉防己碱 /
高效液相色谱法
{{custom_keyword}} /
Key words
Zhongfei mixture /
fangchinoline /
tetraridrine /
HPLC
{{custom_keyword}} /
林能明;张如松;方罗;孔维月.
HPLC测定中肺合剂中防己诺林碱和粉防己碱的含量[J]. 中国药学杂志, 2008, 43(20): 1593-1595
LIN Neng-ming;ZHNG Ru-song;FNG Luo;KONG Wei-yue.
Determination of Fangchinoline and Tetraridrine in Zhongfei Mixture by HPLC [J]. Chinese Pharmaceutical Journal, 2008, 43(20): 1593-1595
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] TAN P，BAO W L，ZHU X R，et al. Treat on 100 cases of advanced non-small cell lung cancer by Zhongfei decoction[J] . Zhejiang J Integr Tradit Chin West Med(浙江中西医结合杂志)，2005，15(1)：12-13.
[2] ZHANG H Y，WU L，HE F G. Clinical observation of Zhongfei liquid in the treatment of pneumonitis induced by postoperative radiotherapy in breast cancer patients[J] . Jiangxi J Tradit Chin Med (江西中医药) ，2006，37(3)：279-280.
[3] HOU G L，ZHANG H Y，WANG C L. Preparation and clinical application of Zhongfei liquid[J] . Jiangxi J Tradit Chin Med (江西中医药) ，2006，37(2)：56-57.
[4] WANG Y Z，MA S L，ZAHNG A Q，et al. Experimental study of the protective effects of Zhongfei decoction on radiation-induced pneumonia in rats[J] . Chin J Radiol Med Prot(中华放射医学与防护杂志)，2007，27(3)： 232-235.
[5] WANG Y Z，MA S L，ZAHNG A Q，et al. The protective effects of Zhongfei decoction on radiation-induced pneumonia in rats[J] . Zhejiang Med J(浙江医学)，2007，29(5)：434-436.
[6] CHEN L Y，ZHOU X F，WEN L，et al. Identification the main components of Zhongfei liquid by TLC[J] . Her Med(医药导报)，2005，24 (12)：945-946.
[7] ZHANG Y，SUN J X，GAO Y L. Pharmacokinetics of tetrandrine ophthalmic solution in the rabbit eye[J] . Chin Hosp Pharm(中国医院药学杂志)，2007，27(3)：306-309.
[8] FENG B M，YE Y，ZHANG H. Determination of tetrandrine in Stephania terandra by HPLC[J] . Chin Pharm (中国药业)，2005，14 (11)：36-37.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
浙江省中医药科技计划(2007CA098)
{{custom_fund}}
