OBJECTIVE To select the optimum preparation process of inclusion complex of genipin-β-cyclodextrin.METHODS The inclusion compound was prepared by the trituration method.The multiple factor and levels experiments were done by orthogonal design with the recovery of inclusion complex and the content of genipin as criteria.RESULTS The optimum entrapment conditions were as follows: the reaction time was 1 h,the molar ratio of genipin to β-cyclodextrin was 1∶1,the solution was water.The formation of compound genipin-β-cyclodextrin was confirmed by infrared spectroscopy,ultraviolet spectra,scanning electron microscopy(SEM),X-ray diffractometry and Phase Solubility Method.CONCLUSION The recovery of inclusion complexm prepared with the optimum inclusion condition was 76.23% and the content of genipin was 16.5 mg·g-1.The stability of genipin was enhanced.Meanwhile,such inclusion complex may be fuller developed into a lot of preparations.
LU Yi;ZHNG Tong;TO Jin-sheng;LIN Min.
Study on Preparation Process of Inclusion Complex of Genipin-β-Cyclodextrin [J]. Chinese Pharmaceutical Journal, 2008, 43(20): 1568-1573
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] OKADA K，SHODA J，KANO M，et al. Inchinkoto，a herbal medicine，and its ingredients dually exert Mrp2/MRP2-mediatedcholeresis and Nrf2-mediated antioxidative action in rat livers[J] . Am J Physiol Gastrointest Liver Physiol，2007，292(5)：1450-1463.
[2] YANG L，AKAO T，KOBASHI K. Purification and characteri zation of a geniposide-hydrolyzing beta-glucosidase from Eubacterium sp. A-44，a strict anaerobe fromhuman feces[J] . Biol Pharm Bull，1995，18(9)：1175-1178.
[3] INAO M，MOCHIDA S，MATSUI A，et al. Japanese herbal medicine Inchin-ko-to as a therapeutic drug for liver fibrosis[J] . J Hepatol，2004 ，41(4)：584-591.
[4] KOO H J，LIM K H，JUNG H J，et al. Anti-inflammatory evaluation of gardenia extract，geniposide and genipin[J] . J Ethnopharmacol，2006，103(3)：496-500.
[5] KOO H J，SONG Y S，KIM H J，et al. Antiinflammatory effects of genipin，an active principle of gardenia[J] . Eur J Pharmacol，2004，495(2-3)：201-208.
[6] TAKEUCHI S，GOTO T，MIKAMI K，et al. Genipin prevents fulminant hepatic failure resulting in reduction of lethality through the suppression of TNF-alpha production[J] . Hepatol Res，2005，33(4)：298-305.
[7] CHANG Y，CHEN S C，WEI H J，et al. Tissue regeneration observed in a porous acellular bovine pericardium used to repair a myocardial defect in the right ventricle of a rat model. [J] . J Thorac Cardiovasc Surg，2005，130(3)：705-711.
[8] SHODA J，MIURA T，UTSUNOMIYA H. Genipin enhances Mrp2(Abcc2)-mediated bile formation and organic anion transport in rat liver[J] . Hepatology，2004，39(1)：167-178.
[9] FU H L，LIANG H Z，LIAO F S. Present condition and perspective of utilization of the geniposidein gardenia jasminoides[J] . Lishizhen Med Mater Med Res(时珍国医国药)，2005，16(1)：54-56.
[10] CHEN H，OUYANG W，JONES M，et al. Preparation and characterization of novel polymeric microcapsules for live cell encapsulation and therapy[J] . Cell Biochem Biophys，2007，47(1)：159-168.
