目的利用HPLC-DAD方法,研究川党参药材的HPLC色谱指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法色谱条件Agilent ZORBAX SB-C₁₈色谱柱(4.6mm×250mm,5μm);乙腈-0.5%醋酸水溶液为流动相进行二元梯度洗脱;柱温30℃;检测波长268nm;流速1mL·min^-1。以党参炔苷为参照物分析了19批不同产地的川党参,采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A进行评价,建立了共有模式,并以相关系数评价指纹的相似性。结果19批川党参样品HPLC指纹图谱的相似度都在80%以上,精密度、稳定性、重复性实验中各共有峰相对保留时间和相对峰面积的RSD均<3%,可以明显区别川党参跟药典收载其他品种。结论本方法操作简便,结果稳定可靠,该法可以作为川党参药材的鉴别和质量控制。

OBJECTIVE To establish the fingerprint of Codonopsis tangshen Oliv.by HPLC-DAD.METHODS The separation was performed on a Agilent ZORBAX SB-C₁₈ column(4.6 mm×250 mm,5 μm)with linear gradient elution.The mobile phase consisted of 0.5% acetic acid solution and acetonitrile as mobile phase at the flow rate of 1 mL·min^-1.The column temperature was at 30 ℃.UV detection wavelength was at 268 nm.By Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A),samples were examined to generate a mean global chromatogram and the similarity of eachchromatogram against the mean global chromatogram was also calculated.RESULTS All the similarity of the 19 samples was more than 80%.The RSD of the relative retention time and relative peak area of the shared peak were lower than 3% in the precision,stability and repetitiveness experiments.The chromatogram of Codonopsis tangshen Oliv.showed drastic differences with the other genuine codonopsis.CONCLUSION The method was simple,reproducible and reliable.It can be used to control the quality of Codonopsis tangshen Oliv.