Simultaneous Determination of Dexamethasone,Testosterone and 6β-OH Testosterone in William's Medium E
LI Liang1，GUAN Zheng1，MA Xin1，JIU Xiang-fei1，LU Wei1，2
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1. Department of Pharmaceutics，School of Pharmaceutical Sciences，Peking University，Beijing 100083，China；2.State Key Laboratory of Natural and Biomimetic Drugs，School of Pharmaceutical Sciences，Peking University，Beijing 100083，China
OBJECTIVE To establish a RP-HPLC method with UV detection for the simultaneous determination of dexamethasone,testosterone and 6β-OH testosterone in William's Medium E,and to investigate in vitro induction of CYP3A by dexamethasone in primary rat hepatocyte culture.METHODS Aglient 1100 HPLC system was used in the drug analysis.The samples were separated on a reversed-phase HPLC column(phenomenex) with the mobile phase consisting of acetonitrile and 0.01 mol·L-1 phosphoric acid solution,using a linear gradient elution program.Ketoconazole was used as internal standard.The flow rate was set at 1.00 mL·min-1.The detection wavelength was at 245 nm and the column temperature was 20 ℃.RESULTS Dexamethasone,testosterone and 6β-OH testosterone had good linear relationship over the range of 53～19 291,161～29 318 and 58～21 091 μg·L-1,respectively.The intra-day and inter-day RSDs were less than 15%.The LOQ in William's Medium E of dexamethasone,testosterone and 6β-OH testosterone were 53.00,161.00 and 58.00 μg·L-1,respectively.CONCLUSION The developed method is sample,accurate and reliable for determining dexamethasone,testosterone and 6β-OH testosterone simultaneously in William's Medium E after induction of CYP3A by dexamethasone in primary rat hepatocyte culture.
LI Ling;GUN Zheng;M Xin;JIU Xing-fei;LU Wei;.
Simultaneous Determination of Dexamethasone,Testosterone and 6β-OH Testosterone in William's Medium E [J]. Chinese Pharmaceutical Journal, 2008, 43(11): 870-873
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