OBJECTIVE To establish a HPLC-UV assay for the determination of icarisideⅠ in blood and tissue samples,and to study its pharmacokinetics and tissue distribution in rats.METHODS IcarisideⅠ was analyzed on a Dikma C18 column with the mobile phase consisting of methnol-0.2% phosphate acid(73∶27) at 1.0 mL·min-1 flow rate and with column temperature at 20 ℃.The wavelength of UV detector was set at 270 nm.A single dose of 10 mg·kg-1 icarisideⅠ was administrated intravascularly in rats.The icariside Ⅰ concentrations in blood and tissues were assayed by HPLC-UV method.The pharmacokinetic parameters of icarisideⅠ were calculated by 3P97 program.RESULTS The concentration-time curve of icarisideⅠ was fitted to a two-compartment model.IcarisideⅠ was remained in heart and muscle in some degree.CONCLUSION The method was convenient,accurate and specific.It was suitable for the study on pharmacokinetis and tissue distribution of icariside Ⅰ.
LIU Zi-chen;XU Ying;CHEN Shi-zhong.
Study on Pharmacokinetics and Tissue Distribution of IcarisideⅠ in Rats by HPLC-UV Assay [J]. Chinese Pharmaceutical Journal, 2008, 43(11): 848-851
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参考文献
[1] LIU T H，WANG Y，WANG B X，et al. Studies on the metabolism of icariin by intestinal bacteria partⅠ： The transformation of icariin by intestinal flora[J] .Chin Tradit Herb Drugs(中草药)，2000，31(11)：834-837.
[2] QIU F，CHEN Y J，KANO Y，et al. Metabolism of orally administered icariin in rats[J] .Acta Pharm Sin(药学学报)，1999，34(3)：222-226.
[3] LENOBLE R，RICHHEIMER S，BAILEY D T，et al. Compositions comprising icarisideⅠ and anhydroicaritin and methods for making the same：PCT，WO 02/13842 A1[P] .2002-02-21.
[4] XIN Z C，YUAN Y M，FU J，et al. Effects of icariin on intracavernosal pressure and systematic arterial blood pressure of rat[J] . Natl Med J Chin(中华医学杂志)，2004，84(2)：142-145.
[5] TIAN L，XIN Z C，LIU W J，et al. Effects of icariin on the erectile function and expression of nitrogen oxide synthase isoforms in corpus cavernosum of arteriogenic erectile dysfunction rat model[J] . Natl Med J Chin(中华医学杂志)，2004，84(11)：954-957.
[6] YE L K，CHEN J M，LIU S H，et al. Pharmacokinetics of icariin in rats[J] .Chin Pharm J(中国药学杂志)，1999，34(1)：33-36.
