摘要
目的建立简单、快速且能同时分离测定刺五加叶中芦丁、金丝桃苷含量的毛细管电泳法。方法采用毛细管电泳法,缓冲体系为0.2mol·L-1NaH2PO4,0.1mol·L-1Borax及双蒸水(10:7:3,pH8.8),熔融石英毛细管柱(25μm×45cm),分离电压为20kV,检测波长为254nm,进样时间为30s,进样高度10cm。结果芦丁、金丝桃苷在125～750,200～1200mg·L-1与峰面积线性关系良好,线性方程分别为:Y=144145ρ+423.8(r=0.9999)及Y=216836ρ-191.57(r=0.9999),样品平均回收率为99.38%及100.39%。结论该方法简单、快速、准确、重现性好,不同生长时期、不同产地的刺五加叶中两成分均存在一定的差异。
Abstract
OBJECTIVE To develop a simple and rapid high-performance capillary electrophoresis(HPCE) coupled with UV detection method for the determination of rutin and hyperin in Acanthopanax senticosus(Rupr.et Maxim) leaves simultaneously.METHODS The buffer was composed of 0.2 mol·L-1 NaH2PO4,0.1 mol·L-1 Borax and double distilled water(10∶7∶3,pH 8.8) solution.Capillary electrophoresis was performed using a 25 μm×45 cm fused silica capillary column.Separation voltage was 20 kV,detection wavelength was set at 254 nm,and the injection time was 30 s at a height of 10 cm.RESULTS The linear range of rutin was from 125～750 mg·L-1,its linear regression equation was Y=144 145ρ+423.87(r=0.999 9),and the average recovery was 99.38%,while the linear range of hyperin was from 200～1 200 mg·L-1,its linear regression equation was Y=216 836ρ-191.57(r=0.999 9),and the average recovery was 100.39%.CONCLUSION This method can be used for the quality control of the Acanthopanax senticosus(Rupr.et Maxim) leaves with good precision.The contents of rutin and hyperin are different in Acanthopanax senticosus(Rupr.et Maxim) from different places and different collection periods.
关键词
刺五加叶 /
高效毛细管电泳 /
芦丁 /
金丝桃苷
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Key words
Acanthopanax senticosus(Rupr. et Maxim) leaves /
HPCE /
rutin /
hyperin
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郑春英;刘松梅;李宏涛;石震华;平文祥.
毛细管电泳法同时检测刺五加叶中的芦丁和金丝桃苷含量[J]. 中国药学杂志, 2008, 43(10): 783-785
ZHENG Chun-ying;LIU Song-mei;LI Hong-to;SHI Zhen-hu;PING Wen-xing.
Simultaneous Determination of Rutin and Hyperin in Acanthopanax senticosus(Rupr. et Maxim) Leaves by High-Performance Capillary Electrophoresis [J]. Chinese Pharmaceutical Journal, 2008, 43(10): 783-785
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参考文献
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脚注
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基金
哈尔滨市科技创新人才研究专项资金项目(2006RFQXS097);黑龙江省教育厅科学技术研究资助项目(11511289);黑龙江大学博士启动基金项目
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