OBJECTIVE To built a sensitive and specific HPLC-UV method for the determination of ondansetron and chlorpromazine simultaneously in rat plasma.METHODS Telmisartan was used as internal standard and the plasma samples were extracted with ethyl acetate.The separation was carried out on a C8 column(4.6 mm×250 mm,5 μm) using a mobile phase consisting of methanol-water(70∶30) delivered at 1 mL·min-1.The rats were given with ondansetron,chlorpromazine or ondansetron combined with chlorpromazine respectively after 12 h fasting or feeding with high protein food.The plasma concentration was determinated by the HPLC-UV method and the pharmacokinetic parameters were analyzed.RESULTS The ρmax,t1/2,MRT and AUC0→t and AUC0→∞ of ondansetron were elevated after combining with chlorpromazine,and the ρmax,t1/2,MRT and AUC0→t,AUC0→∞ of chlorpromazine had no obviously change.The high protein food increased the ρmax,AUC0→t and AUC0→∞ of the ondansetron and decreased the ρmax,AUC0→t and AUC0→∞ of the chlorpromazine.CONCLUSION When the ondansetron was combined with chlorpromazine,the pharmacokinetics of ondansetron was changed significantly.High protein food can make significant effect on the pharmacokinetic parameters of ondansetron and chlorpromazine.
SHEN Yu-ln;FENG Fng.
Study on Pharmacokinetic Interaction of Ondansetron and Chlorpromazine in Rat [J]. Chinese Pharmaceutical Journal, 2008, 43(10): 771-775
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