OBJECTIVE To compare the syringin content in cell cultures with that in botanical drug of Saussurea involucrata.METHODS A HPLC-DAD method was developed as follows:chromatographic separation was achieved on an Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm) at 25 ℃ and the mobile phase was a mixture of acetonitrile-water(10∶90) detected at 220 nm at a flow rate of 1.0 mL·min-1.RESULTS Compared with the botanical drug,the content of syringin in cells was improved 20.96 times by liquid culture and 55.50 times by solid culture.CONCLUSION It has a good prospect to acquire syringin by plant cell culture.
LI Yn;GUO Shun-xing;WNG Chun-ln;WU Li-qin;XIO Pei-gen.
Comparison of Syringin Content Between Cell Cultures and Botanical Drug of Saussurea involucrata[J]. Chinese Pharmaceutical Journal, 2008, 43(10): 737-738
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参考文献
[1] Ch.P (2005) Vol I (中国药典2005年版. 一部)[S] . 2005： 36-37.
[2] CHIO J，SHIN K M，PARK H J，et al. Anti-inflammatory and antinociceptive effects of sinapyl alcohol and its glucoside syringin[J] . Planta Med，2004，70(11)：1027-1032.
[3] CHO J Y，NAM K H，KIM A R，et al. In-vitro and in-vivo immunomodulatory effects of syringin[J] . J Pharm Pharmacol，2001，53(9)：1287-1294.
[4] KAPIL A，SHARMA S. Immunopotentiating compounds from Tinospora cordifolia[J] . J Ethnopharmacol，1997，58：89-95.
[5] MIAO A D，SUN D J，PENG Y，et al. RP-HPLC determination of syringin in Xuelian injection [J] . Chin J Pharm Anal(药物分析杂志)，2003，23(4)： 289-290.
[6] MIAO A D，SUN D J，PENG Y，et al. Study on HPLC fingerprint of Xuelian injection [J] . China J Chin Mater Med (中国中药杂志)，2004，29(11)： 1044-1046.
