摘要
目的研究鉴定2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(SBGC)在Beagle犬体内的代谢行为。方法选择健康Beagle犬3只单剂量0.4g·kg-1灌胃SBGC,于给药后60min后肢静脉取血,3000r·min-1离心10min,分取血浆,将血浆经SPE-C18固相柱纯化后,采用LC/MSn方法对血浆中的待测的代谢产物进行总离子监测(TIM)和多级全扫描质谱分析(MSn)。结果在Beagle犬血浆中检测到母体成分SBGC和4种代谢产物。结论SBGC在Beagle犬体内主要与葡萄糖醛酸相结合形成II相代谢产物。
Abstract
OBJECTIVE To investigate the major metabolites of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Beagle dogs.METHODS The blood samples were collected from vein after a single dose of 0.4 mg·kg-1 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside.The plasma samples which were separated after centrifugation(3 000 r·min-1)were purified by a SPE column(Sep-C18)and analyzed with LC/MSn for potential metabolites.RESULTS 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside and its four metabolites were first identified in Beagle dogs plasma.CONCLUSION The major metabolic pathway of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Beagle dogs is conjugating with glucuronic acid,forming phase Ⅱ metabolites.
关键词
2 /
3 /
5 /
4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 /
Beagle犬 /
代谢 /
高效液相-质谱联用
{{custom_keyword}} /
Key words
2 /
3 /
5 /
4'-tetrahydroxystilbene-2-O-β-D-glucoside /
Beagle dog /
metabolites /
LC/MSn
{{custom_keyword}} /
王福刚;刘斌;王伟;石任兵;陈玉平;王玉霞.
LC/MSn鉴定2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在Beagle犬体内的主要代谢产物[J]. 中国药学杂志, 2008, 43(09): 710-712
WNG Fu-gng;LIU in;WNG Wei;SHI Ren-ing;CHEN Yu-ping;WNG Yu-xi.
Identification of Major Metabolites of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside in Beagle Dog Plasma by Liquid Chromatography/ion Trap Mass Spectrometry [J]. Chinese Pharmaceutical Journal, 2008, 43(09): 710-712
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] SHENG Z，XUE G.Growth inhibitoby effect of stilbenoids on lung cancer cell lines[J] . Acta Acad Med Shanghai（上海医科大学学报），1998，25（5）：330.
[2] LIU H C，CHEN W S. Sdudies on antioxidant activity of 2，3，5，4′-tetrahydroxystilbene-2-O-β-D-glucoside of water-soluble comonent form polygonum multiflorum in vitro[J] .J Pharm Practice（药学实践杂志），2000，18（4）：232-237.
[3] RYU G，JU J H，PARK Y J，et al. The radical scavenging effects of stilbene glycosides from Ploygonum multiflorum[J] . Archieves Phamacal Res，2002，25（5）：636-639.
[4] XIAO K，XUAN L J，XU Y M，et al. Novel stilbene glycosides from Polygonum multiflorum[J] . Acta Botanica Sin，2002，44（12）：1491-1494.
[5] GU J K，ZHONG D F，CHEN X Y. Analysis of O-glucuroside conjugates in urine by electrospray ion trap mass spectrometry[J] . Fresenius J Anal Chen，1999，365（12）：553-558.
[6] CHEN X Y，ZHONG D F，JIANG H. Characterization of some glucuroside conjugates by eelectrospray Ion trap mass spectrometry[J] . Acta Pharm Sin（药学学报），1998，33(11)：849-854.
[7] APPOLPNOVA S A，SHPAK A V，SEMENOV V A. Liquid chromatography electrospray ionization ion trap mass spectrometry for analysis of mesocarb and its metabolites in human urine[J] .J Chromatogr B，2004，800：281-289.
[8] YANG X W，HAO M R，HATTORI MASAO （JAPANESE）. Analysis on matabolism of Chinese Herbal Ingredients（中药成分代谢分析）[M] . Beijing： Chinese Medical and Pharmaceutical Science Press，2003：95-96.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
“十一五”国家科技支撑计划(2006BAI08B03-04);教育部科学技术研究重点项目(105029);国家自然科学基金资助项目(90409011,30672598);北京市自然科学基金资助项目(7061002)
{{custom_fund}}
