摘要
目的建立测定小鼠血浆中G蛋白抑制性多肽-27(GCIP-27)浓度的放射性核素(125I)示踪测定法。方法125I-GCIP采用Iodogen法标记,血浆中GCIP的浓度采用同位素示踪法结合三氯醋酸(TCA)沉淀法或低相对分子质量SDS-PAGE电泳法测定,并比较其测定结果。结果TCA沉淀法和电泳法同时对照测定60份GCIP血浆样品,结果呈良好的相关性,r=0.9554。2种方法最低检测浓度均为2.0μg·L-1,日内、日间RSD均小于10%,血浆在3.75~480μg·L-1内均呈良好的相关性,相关系数分别为r=0.9977和0.9964,血浆中GCIP的平均回收率分别为(92.72±4.55)%和(91.77±5.21)%。结论同位素示踪法与沉淀法或电泳法结合,方法稳定、可靠、灵敏度较高,两法相辅相成,适于GCIP-27血药浓度测定。
Abstract
OBJECTIVE To develop an isotope tracing method for the pharmacokinetics of GCIP-27 in mice.METHODS 125I-GCIP was prepared by Iodogen method.Isotope tracing method combined with trichloroacetic acid(TCA)precipitation and low molecular weight SDS-PAGE method were used to determine GCIP-27 in mice plasma.RESULTS TCA method and SDS-PAGE method were used to measure the concentration of 60 mouse plasma samples.There was a good linear correlation(r=0.955 4)between the two methods.Within-day and between-day precision were less than 10%.Their linearities were determined in the range of 3.75~480 μg·L-1 in plasma(r=0.997 7 and 0.996 4,respectively).The average recoveries of the two methods of GCIP were(92.72±4.55)and(91.77±5.21)%,respectively.CONCLUSION Both the methods are stable,reliable and sensitive.They are suitable for the determination of GCIP concentration in mice plasma.
关键词
G蛋白抑制性多肽-27 /
同位素标记示踪法 /
TCA沉淀法 /
低分子量蛋白电泳法
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Key words
GCIP-27 /
isotope tracing method /
TCA /
SDS-PAGE
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王秀琴;李晓辉;张海港;杨丹莉.
G蛋白抑制性多肽-27药动学测定方法的建立[J]. 中国药学杂志, 2008, 43(09): 692-695
WNG Xiu-qin;LI Xio-hui;ZHNG Hi-gng;YNG Dn-li.
Development of on Isotope Tracing Method for Pharmacokinetics of GCIP-27 [J]. Chinese Pharmaceutical Journal, 2008, 43(09): 692-695
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参考文献
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脚注
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基金
重庆市自然科学基金重点攻关项目(20048256、20027537、20010725)
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