G蛋白抑制性多肽-27药动学测定方法的建立

王秀琴;李晓辉;张海港;杨丹莉

中国药学杂志 ›› 2008, Vol. 43 ›› Issue (09) : 692-695.

中国药学杂志 ›› 2008, Vol. 43 ›› Issue (09) : 692-695.
论著

G蛋白抑制性多肽-27药动学测定方法的建立

  • 王秀琴,;李晓辉;张海港;杨丹莉
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Development of on Isotope Tracing Method for Pharmacokinetics of GCIP-27

  • WANG Xiu-qin1,2,LI Xiao-hui1*,ZHANG Hai-gang1,YANG Dan-li1
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摘要

目的建立测定小鼠血浆中G蛋白抑制性多肽-27(GCIP-27)浓度的放射性核素(125I)示踪测定法。方法125I-GCIP采用Iodogen法标记,血浆中GCIP的浓度采用同位素示踪法结合三氯醋酸(TCA)沉淀法或低相对分子质量SDS-PAGE电泳法测定,并比较其测定结果。结果TCA沉淀法和电泳法同时对照测定60份GCIP血浆样品,结果呈良好的相关性,r=0.9554。2种方法最低检测浓度均为2.0μg·L-1,日内、日间RSD均小于10%,血浆在3.75~480μg·L-1内均呈良好的相关性,相关系数分别为r=0.9977和0.9964,血浆中GCIP的平均回收率分别为(92.72±4.55)%和(91.77±5.21)%。结论同位素示踪法与沉淀法或电泳法结合,方法稳定、可靠、灵敏度较高,两法相辅相成,适于GCIP-27血药浓度测定。

Abstract

OBJECTIVE To develop an isotope tracing method for the pharmacokinetics of GCIP-27 in mice.METHODS 125I-GCIP was prepared by Iodogen method.Isotope tracing method combined with trichloroacetic acid(TCA)precipitation and low molecular weight SDS-PAGE method were used to determine GCIP-27 in mice plasma.RESULTS TCA method and SDS-PAGE method were used to measure the concentration of 60 mouse plasma samples.There was a good linear correlation(r=0.955 4)between the two methods.Within-day and between-day precision were less than 10%.Their linearities were determined in the range of 3.75~480 μg·L-1 in plasma(r=0.997 7 and 0.996 4,respectively).The average recoveries of the two methods of GCIP were(92.72±4.55)and(91.77±5.21)%,respectively.CONCLUSION Both the methods are stable,reliable and sensitive.They are suitable for the determination of GCIP concentration in mice plasma.

关键词

G蛋白抑制性多肽-27 / 同位素标记示踪法 / TCA沉淀法 / 低分子量蛋白电泳法

Key words

GCIP-27 / isotope tracing method / TCA / SDS-PAGE

引用本文

导出引用
王秀琴;李晓辉;张海港;杨丹莉. G蛋白抑制性多肽-27药动学测定方法的建立[J]. 中国药学杂志, 2008, 43(09): 692-695
WNG Xiu-qin;LI Xio-hui;ZHNG Hi-gng;YNG Dn-li. Development of on Isotope Tracing Method for Pharmacokinetics of GCIP-27 [J]. Chinese Pharmaceutical Journal, 2008, 43(09): 692-695

参考文献

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基金

重庆市自然科学基金重点攻关项目(20048256、20027537、20010725)

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