摘要
目的采用液相色谱-质谱联用法鉴定大鼠口服槲皮素单体后血浆中的代谢产物。方法血浆样品经过2mol·L-1盐酸(甲醇-水)水解后,进行色谱分离,采用液相色谱-质谱联用仪检测,大气压电喷雾离子源(EPI)负离子条件下进行扫描,鉴定大鼠给药槲皮素(100mg·kg-1)单体后血浆中代谢产物的结构。结果通过与标准物紫外谱图比较,结合质谱仪扫描结果分析,在大鼠给药槲皮素(100mg·kg-1)单体后血浆中鉴定出槲皮素(m/z→301.0)和异鼠李素(m/z→314.9)两种代谢产物结构。结论采用盐酸酸水解的方法可快速、有效地鉴定出大鼠血浆中槲皮素代谢产物,为进行含槲皮素中药及其复方的药动学研究提供参考。
Abstract
OBJECTIVE To determine the metabolites of quercetin in rat plasma after an oral administration by Liquid Chromatography-Mass Spectrometry(LC-MS).METHODS The rats were administrated with quercetin monomer solution(100 mg·kg-1)oral and the blood sample were collected.Quercetin and isorhamnetin were extracted from rat plasma after acid hydrolysis using 2 mol·L-1 HCl in aqueous methonl.After acid hydrolysis,the analytes of interest were separated on a SUPELCOSILTMC18 column.The mobile phase was methonl-acetonitrile-1% acetic acid(20:30:50)with a flow rate of 0.8 mL·min-1.A Bruker Mass Spectrometry equipped with an Atmospheric Pressure Ionization-Electrospray(EPI) source was used as detector and was operated in negative ion mode.RESULTS Quercetin and isorhamnetin were identified using the standard spectrum and the results of mass spectrometry,i.e.m/z→301.0 for quercetin and m/z→314.9 for isorhamnetin.CONCLUSION Acetic acid hydrolysis was a fast feasible method and could be used to identify the quercetin metabolites in rat plasma.And this method could be a guidance for pharmacokinetics studie upon Traditional Chinese Medicines.
关键词
槲皮素 /
异鼠李素 /
液相色谱-质谱联用法 /
代谢物
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Key words
quercetin /
isorhamnetin /
LC-MS /
metobolites
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陈峰;符乃光;任守忠;魏娜;张俊清.
液相色谱-质谱联用法测定大鼠血浆中的槲皮素代谢物[J]. 中国药学杂志, 2008, 43(03): 225-227
CHEN Feng;FU Ni-gung;REN Shou-zhong;WEI N;ZHNG Jun-qing.
Determination of Quercetin Metabolites in Rat Plasma by Liquid Chromatography-Mass Spectrometry [J]. Chinese Pharmaceutical Journal, 2008, 43(03): 225-227
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参考文献
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脚注
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基金
海南省自然科学基金资助项目(30626)
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