1.Second Affiliated Hospital，College of Medicine，Zhejiang University，Hangzhou 310009，China； 2. Institute of Pharmaceutics，Zhejiang University，Hangzhou 310031，China
OBJECTIVE To investigate the possibility of percutaneous transport of Cinnamon extract in β-cyclodextrin(β-CD) composite ethosomes.And to determine the effect of the concentration of ethanol and penetration enhancers on the permeation rate.METHODS β-CD composite ethosomes was prepared by injection method.With the skin of SD male rat,the penetration experiments in vitro were performed with modified Franz diffusion cells.The penetration concentrations of β-CD composite ethosomes was determined by HPLC.RESULTS The steady permeation rate of β-CD composite ethosomes with 20%,30%,40% and 50% ethanol were(5.06±1.29),(8.08±3.76),(10.05±1.76) and(17.89±0.15)μg·h-1·cm-2 respectively.And it was 1.01,1.62,2.01 and 3.18 times as that of(5.00±0.75)μg·h-1·cm-2β-CD aqueous solution.Penetration enhancer of DMSO and 1,2-trimethylene glycol increased the permeability by 1.50 and 1.83 times,compared with that of β-CD composite aqueous solution.CONCLUSION Ethosomes can improve β-CD inclusion permeation,and increase the skin accumulation of the drug.
XU Dong-hng;XU Xing;WN Wei-feng;WEI Xio-hong.
Study on Percutaneous Permeability of Cinnamon Extract in β-Cyclodextrin Composite Ethosomes[J]. Chinese Pharmaceutical Journal, 2008, 43(03): 196-198
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参考文献
[1] MEI Q X，BI H X. Handbook of Modem Pharmacology of Traditional Chinese Medicine(现代中药药理手册)[M] . Beijing：China Press of Traditional Chinese Medicine，1998：276.
[2] ZHU W F，ZHANG Y H，LIU H N，et al. Experimental study on percutaneous absorption of cinnamic acid [J] .Chin Tradit Pat Med（中成药），2003，25 (6)：443-445.
[3] ZHAO H R，YANG Y G，HE Y，et al. Characteristics of particles of Cortex cinnamomi Ultra-fine powder and its transdermal effect in vitro[J] .Chin Tradit Pat Med（中成药），2002，24(9)：653-656.
[4] HAO M R，LI C L.Studies on transdermal absorption of goupibabu plaster in vitro using HPLC[J] .Chin Tradit Herb Drugs（中草药），1998，29(1)：23-25.
[5] Institute of Medicinal Plant Developments and Institute of Materia Medica of Chinese Academy Medical Science.Zhong Yao Zhi(中药志) (V)[M] . Beijing：People’s Medical Publishing House，1994：388.
[6] SONG H T，GUO T，ZHAO M H，et al. Study on the physicochemical properties of Cinnamon oil beta-cyclodextrin inclusion complex[J] .Chin J Hosp Pharm(中国医院药学杂志)，2003，23(1)：12-14.
[7] SONG H T，GUO T，YAN X T，et al. Studies on the preparation of cinnamon oil-β-cyclodextr in inclusion complex[J] .Chin Tradit Herb Drugs（中草药），2000，31(11)：818-820.
[8] DAYO A，PING Q N，LIU G J. Enhancing effect of eucalyptus oil，and its β-cyclodextrin complex on the permeation of 5-fluorouracil through excised rat skin [J] .J Chin Pharm Univ(中国药科大学学报)，1996，27（2）：77-82.
[9] GODIN B，TOUITOU E. Ethosomes： new prospects in transdermal delivery[J] .Crit Rev Ther Drug Carrier Syst，2003，20(1)：63-102.
[10] TOUITOU E，DAYAN N，BERGELSON L，et al. Ethosomes - novel vesicular carriers for enhanced delivery： characterization and skin penetration properties[J] .J Controlled Release，2000，65(3)：403-418.
[11] XU D H，ZHANG Q，FENG X，et al. Synergistic effects of ethosomes and chemical enhancers on enhancement of naloxone through human skin[J] . Pharmazie，2007，62(4)：316-318.
[12] FENG X，XU D H，XU X，et al. Study on percutaneous permeation of naloxone ethosomes[J] .Chin J Mod Appl Pharm(中国现代应用药学杂志)，2008，25(1)：65-67.
[13] RAO Y F，LI F，LIANG W Q. Study on transdermal permeation and skin accumulation of finasteride ethosomes[J] .Chin Pharm J（中国药学杂志），2004，39：139-172.
[14] LU B. New Techniques and New Dosage Forms of Drugs (药物新剂型与新技术) [M] . 2nd.Beijing： People's Medical Publishing House，2005：35.
[15] GUO T，SONG H T，YAN X T，et al. Study on the stability of cinnamon oil beta-cyclodextrin inclusion complex[J] .China J Chin Mater Med(中国中药杂志)，2000，25（7）：411-413
[16] LI G W，JIANG H Y，XU D H，et al. Optimizing the inclusion technology of cinnamon volatile oil-β-cyclodextrin inclusion complex[J] .Chin Pharm(中国药房)，2007，18（21）：1623-1625.
