Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode
LI Wen-long1,CHEN Jun-hui1,2,YIN Yue-fen1,2,WU Feng-qi1,YANG Bai-juan1,YANG Huang-hao1,WANG Xiao-ru1,3
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1.Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines,First Institute Oceanography of SOA,Qingdao 266061;2. College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266006,China;3. Department of Chemistry and Key Laboratory of Analytical Science of the MOE,College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China
OBJECTIVE To develop MEKC and MEEKC modes for the determination of gentiopicroside and loganic acid in extracts of Radix Gentianae. METHODS The analyte was extracted from Radix Gentianae samples by accelerated solvent extraction, and the extraction conditions were optimized. Separation and determination were carried out on a bared fused silica capillary(50 cm×75 μm) with corresponding buffer. The run voltage of MEKC and MEEKC were 30kV and 22 kV respectively. Detection wavelength of DAD was at 238 nm and column temperature was 25 ℃. RESULTS The developed ASE-HPCE method was simple and reliable for the determination of gentiopicroside and loganic acid in Radix Gentianae samples with a broad linear dynamic range, a recovery range of 96.3%~105.16%,and the detection limit was below 10 mg·L-1. The contents of gentiopicroside and loganic acid in six samples from different regions were determined by the developed method in two modes. T-test value indicated that the determined contents of gentiopicroside and loganic acid by MEKC and MEEKC were consistent. CONCLUSION The method is simple,accurate,rapid and with good reproducibility.It can be used to determine active components in Radix Gentianae.
LI Wen-long;CHEN Jun-hui;YIN Yue-fen;WU Feng-qi;YNG i-jun;YNG Hung-ho;WNG Xio-ru;.
Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode [J]. Chinese Pharmaceutical Journal, 2007, 42(22): 1736-1740
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