MEKC和MEEKC两种模式测定龙胆中龙胆苦苷和马钱子苷酸的含量

李文龙;陈军辉;殷月芬;吴凤琪;杨佰娟;杨黄浩;王小如

中国药学杂志 ›› 2007, Vol. 42 ›› Issue (22) : 1736-1740.

中国药学杂志 ›› 2007, Vol. 42 ›› Issue (22) : 1736-1740.
论著

MEKC和MEEKC两种模式测定龙胆中龙胆苦苷和马钱子苷酸的含量

  • 李文龙;陈军辉,;殷月芬,;吴凤琪;杨佰娟;杨黄浩;王小如,
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Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode

  • LI Wen-long1,CHEN Jun-hui1,2,YIN Yue-fen1,2,WU Feng-qi1,YANG Bai-juan1,YANG Huang-hao1,WANG Xiao-ru1,3
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文章历史 +

摘要

目的建立胶束电动毛细管色谱(MEKC)和微乳液毛细管电动色谱(MEEKC)分析龙胆药材中龙胆苦苷和马钱子苷酸含量的方法。方法采用加速溶剂萃取法(ASE)对龙胆药材进行提取,萃取温度:100℃,压力:9.65MPa,萃取时间:10min。采用未涂层熔融石英毛细管(内径75μm,有效长度50cm)。分别考察了两种分离模式下电泳介质的构成和电泳过程中的各操作参数对样品分离过程的影响,优化了MEKC和MEEKC的分析条件,在各自对应的缓冲液体系下,MEKC和MEEKC分离电压分别为30和22kV,柱温均为25℃,检测波长均为238nm。结果在选定的工作条件下,龙胆苦苷和马钱子苷酸与其他组分达到了基线分离,两种成分的浓度与其响应信号值之间具有较好的线性相关性,加标回收率在96.3%~105.1%之间,检测限均低于10mg·L-1,对6处不同产地的龙胆药材进行了分析,并对测定结果进行了t检验,结果表明,两种模式下,测定结果之间不存在显著性差异,而不同产地的龙胆药材的龙胆苦苷和马钱子苷酸含量之间存在较大差异。结论本方法简便,准确,快速,重现性较好,可用于龙胆药材有效成分的含量测定和质量控制。

Abstract

OBJECTIVE To develop MEKC and MEEKC modes for the determination of gentiopicroside and loganic acid in extracts of Radix Gentianae. METHODS The analyte was extracted from Radix Gentianae samples by accelerated solvent extraction, and the extraction conditions were optimized. Separation and determination were carried out on a bared fused silica capillary(50 cm×75 μm) with corresponding buffer. The run voltage of MEKC and MEEKC were 30kV and 22 kV respectively. Detection wavelength of DAD was at 238 nm and column temperature was 25 ℃. RESULTS The developed ASE-HPCE method was simple and reliable for the determination of gentiopicroside and loganic acid in Radix Gentianae samples with a broad linear dynamic range, a recovery range of 96.3%~105.16%,and the detection limit was below 10 mg·L-1. The contents of gentiopicroside and loganic acid in six samples from different regions were determined by the developed method in two modes. T-test value indicated that the determined contents of gentiopicroside and loganic acid by MEKC and MEEKC were consistent. CONCLUSION The method is simple,accurate,rapid and with good reproducibility.It can be used to determine active components in Radix Gentianae.

关键词

加速溶剂萃取 / 胶束电动毛细管色谱 / 微乳液毛细管电动色谱 / 龙胆 / 龙胆苦苷 / 马钱子苷酸

Key words

accelerated solvent extraction / MEKC / MEEKC / Radix Gentianae / gentiopicroside / loganic acid

引用本文

导出引用
李文龙;陈军辉;殷月芬;吴凤琪;杨佰娟;杨黄浩;王小如 . MEKC和MEEKC两种模式测定龙胆中龙胆苦苷和马钱子苷酸的含量[J]. 中国药学杂志, 2007, 42(22): 1736-1740
LI Wen-long;CHEN Jun-hui;YIN Yue-fen;WU Feng-qi;YNG i-jun;YNG Hung-ho;WNG Xio-ru;. Determination of Gentiopicroside and Loganic Acid in Radix Gentianae by MEKC and MEEKC Mode [J]. Chinese Pharmaceutical Journal, 2007, 42(22): 1736-1740

参考文献

[1] YANG S B,WANG C.The reserch of chemical constituent and pharmaceutical action of gentianaceae[J] .Acta Chin Med Pharmacol(中医药学报),2005,33(6):54-56. [2] YANG W X,ZHOU L,GENG H L,et al. A survey of study of chemical components of medicinal plants of Gentianaceae[J] .Acta Bot Boreal Occident Sin(西北植物学报),2003,23(12):2235-2240. [3] LIANG X Y,TIAN Q,LI C F,et al. Gentiopicroside content in violet Gentian by LC-MS and HPLC[J] .Chin Tradit Pat Med(中成药),2006,28(4):548-552. [4] LU Y C,LIN P C,JI L J,et al. Determination of three eficacious component in different parts of radix gentianae by HPLC[J] .Chin Trad Pat Med(中成药),2005,27(11):1356-1357. [5] CAO Y N,SUN Y,LI L,et al. HPCE Determination of Gentiopicroside in Radix Gentianae[J] .Chin J Pharm Anal(药物分析杂志),2005,25(1):81-83. [6] MOU S F,LIU Y J. The principle and appl ication of accelerated solvent extraction[J] . Mod Sci Instru(现代科学仪器),2001,3:18-20. [7] MOU S F,LIU K N,YANY,et al. Accelerated solvent extration(ASE) and application on enviromental aalysis[J] .Envir Chem,1997,16(4):387-391.

基金

国家自然科学基金重点项目(20235020);青岛“2004将才计划”(04-3-JJ-11);崂山区校区共建生物医药研发测试中心资助(LS-05-KJZX-76)

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