目的建立四季青药材的HPLC指纹图谱,为该药材的质量评价提供实验依据。方法采用高效液相色谱技术,ZorbaxSb C18柱(4.6mm×250mm,5μm),1%冰醋酸(含0.2%三乙胺)-乙腈梯度洗脱,检测波长为280nm,柱温为30℃,流速为1.0mL·min-1。结果指纹图谱研究和含量测定方法学考察的各项参数均符合有关规定,以系统聚类分析将样品分为两类,每类中各批样品的相似度均大于0.85。结论HPLC指纹图谱的建立为四季青药材的质量评价提供了实验依据。
Abstract
OBJECTIVE To establish a HPLC-FP for the determination of Folium Ilicis Purpurea. METHODS HPLC analysis was performed on a Zorbax Sb C18 column (4.6 mm×250 mm,5 μm),the mobile phase used in gradient elution consisted of 1% acetic acid solution (including 0.2% triethylamine) and acetonitrile, with the flow rate of 1.0 mL·min-1. The column temperature was 30 ℃ and the wavelength was 280 nm. RESULTS 14 Common peaks were obtained in 20 batches of samples. The samples were divided into two groups by hierarchical clustering analyses. The similarity of each fingerprint within one group was higher than 0.85. CONCLUSION The established method can be used for quality control of Folium Ilicis Purpurea.
关键词
四季青 /
高效液相色谱指纹图谱 /
含量测定
{{custom_keyword}} /
Key words
Folium Ilicis Purpurea /
HPLC-fingerprint /
determination of protocatechuic acid
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] XU G J, HE H X, XU L S. Pharmacognosy of China (中国药材学)[M] . Beijing: The Medical Science and Technology Press of China ,1996:893.
[2] ZHAO H R, WANG M S, ZHOU G P. Compounds from Ilex purpurea Hassk[J] .China J Chin Mater Med（中国中药杂志）,1993,18(4):226-228.
[3] XIE J B, LI P. Studies on phenolic acids from Ilex purpurea Hassk[J] .J China Pharm Univ(中国药科大学学报), 2002，33 (1)：76-77.
[4] LIAO L P, BI Z M, LI P, et al. A novel phenolic compound from leaves of Ilex purpurea Hassk[J] .Chem Ind Forest Prod(林产化学与工业) ,2005 ,3 (25): 13-15.
[5] LIAO L P, BI Z M, LI P, et al. Triterpenoids from leaves of Ilex purpurea[J] . Chin J Nat Med(中国天然药物), 2005,3(6):344-346.
[6] MIAO S, BI Z M, LI P. Simultaneous determination of five phenolic aicds from leaf of lexpurpurea by HPLC[J] . J China Pharm Univ (中国药科大学学报), 2003,35(2):127-130.
[7] XIE J B, BI Z M, LI P. HPLC-ELSD Determination of triterpenoids and triterpenoid saponins in Ilex purpurea leaves[J] . Acta Pharm Sin (药学学报), 2003,38(7):534-536.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
博士后科学基金(2004035072)
{{custom_fund}}
