摘要
目的对重组人干扰素α-2b(IFNα-2b)干粉吸入剂的药效学进行评价。方法以IFNα-2b诱导的2′,5′-寡腺苷酸合成酶(OAS)为替代指标,建立了一种灵敏的、半定量的逆转录-聚合酶链反应(RT-PCR)法测定OAS两种亚型OAS1和OAS2在外周血单个核细胞中的mRNA水平。结果与市售注射剂相比较,自制IFNα-2b干粉吸入剂诱导的OAS1的mRNA水平相当,但OAS2的mRNA水平更高。结论重组人干扰素α-2b干粉吸入剂有望成为一种非侵害性的、依从性良好的、发挥全身性疗效的给药途径。
Abstract
OBJECTIVE To evaluate the pharmacodynamic effects of recombinant human interferon α-2b (IFN α-2b) inhalation powder.METHODS 2′,5′-Oligoadenylate synthetase (OAS) was used as a surrogate marker for IFN α-2b induction,and a sensitive and semi-quantitative method was established by use of reverse transcription-polymerase chain reaction (RT -PCR) for the determination of OAS1 and OAS2 forms of OAS mRNA in peripheral blood mononuclear cells .RESULTS OAS1 mRNA concentration was equivalent after IFN α-2b was administrated by injection and inhalation,but the later administration route resulted in a higher OAS2 mRNA level.CONCLUSION IFN α-2b inhalation powder is promising to be a non-invasive systemic delivery route with excellent compliance.
关键词
干扰素α-2b /
干粉吸入剂 /
药效作用 /
RT-PCR /
寡腺苷酸合成酶
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Key words
interferon α-2b /
inhalations powder /
pharmacodynamic effects /
RT-PCR /
2′ /
5′-oligoadenylate synthetase
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江荣高;王立青;刘衡;王春龙.
RT-PCR法评价干扰素α-2b干粉吸入剂的药效学[J]. 中国药学杂志, 2007, 42(22): 1692-1695
JING Rong-go;WNG Li-qing;LIU Heng;WNG Chun-long.
Pharmacodynamic Evaluation of Interferon α-2b Inhalation Powder by RT-PCR [J]. Chinese Pharmaceutical Journal, 2007, 42(22): 1692-1695
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参考文献
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脚注
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