摘要
目的建立了测定中药材人参中多菌灵农药残留量方法。方法采用RP-HPLC法,Diamonsil C18色谱柱。流动相为甲醇-四氢呋喃-水(43∶2∶55),检测波长为277 nm。结果平均回收率人参茎叶83.94%,RSD为2.42%(n=6);平均回收率人参根87.38%,RSD为2.68%(n=6)。多菌灵在1.926～192.6 ng时,r=1.000 0,呈良好的线性关系。结论本方法简便、快速、适合同类中药材中多菌灵农药残留量测定与安全监控。
Abstract
OBJECTIVE To establish a HPLC method for the determination of residual carbendazim in Chinese Traditional Medicine of ginseng.METHODS The mobile phase constituted of methanol-tetrahydrofuran-water(43∶2∶55).A Diamonsil C18 column was used,and carbendazim was determined at the detection wavelength of 277 nm.RESULTSThe averrage recovery and RSD of ginseng stems and leaves were 83.94% and 2.42%(n=6),respectively.The averrage recovery and RSD of ginseng radix were 87.38% and 2.68%(n=6),respectively.The standard curve of carbendazim was linear in the range of 1.926～192.6 ng(r=21.000 0).CONCLUSION This method is simple,rapid and reliable.It is suitable for the determination and safe control of residual carbendazim in this kind of Chinese Traditional Medicine.
关键词
人参 /
多菌灵 /
农药残留 /
高效液相色谱法
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Key words
Ginseng /
carbendazim /
residual pesticide /
HPLC
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彭方;田金改;金红宇;杜庆鹏.
高效液相色谱法测定人参中多菌灵农药残留含量[J]. 中国药学杂志, 2007, 42(06): 475-477
PENG Fng;TIN Jin-gi;JIN Hong-yu;DU Qing-peng.
Determination of Residual Carbendazim in Chinese Traditional Medicine of Ginseng by HPLC [J]. Chinese Pharmaceutical Journal, 2007, 42(06): 475-477
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参考文献
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脚注
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基金
国家科技部科研院所社会公益项目(2004DAB17039)
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