摘要
目的探讨戊地昔布对小鼠肠癌clone 26细胞的生长抑制作用及机制。方法用噻唑蓝(MTT)法检测戊地昔布对clone 26细胞生长的作用。用流氏细胞仪检测clone 26细胞凋亡率和细胞周期分布。用Western blot检测clone 26细胞caspase-3,PCNA和COX-2的表达。结果①戊地昔布可抑制clone 26细胞生长并呈时间和浓度依赖性。②50～400μmol·L-1戊地昔布可明显提高clone 26细胞的凋亡率,从对照的3.1%提高到4.4%～11.0%。给予戊地昔布后,细胞的增殖指数,S期的细胞比例和G2/M期细胞有下降趋势,但只有在400μmol·L-1戊地昔布组时才有统计学意义。③给予戊地昔布后,细胞PCNA的表达降低,caspase-3的表达升高,COX-2的表达没有变化。结论戊地昔布通过诱导凋亡和细胞周期停滞而抑制clone 26细胞生长,小剂量时主要与凋亡有关,大剂量时与诱导凋亡和细胞周期停滞有关。Caspase-3参与戊地昔布诱导的凋亡。
Abstract
OBJECTIVE To study the effect and mechanism of valdecoxib on the proliferation of colorectal cancer clone 26 cell.METHODS MTT assay was used to study the inhibitory effect of valdecoxib on the cancer cell growth.Flow cytometry was used to observe the effect of valdecoxib on proliferation,apoptosis and the cell cycle distribution.Western blot was used to determine the expression of caspase-3,PCNA and COX-2.RESULTS ①Valdecoxib significantly inhibited the proliferation of clone 26 cell in a time-and dose-dependent fashion.②The apoptosis rate was increased from 3.1% in control group to 4.4 %～11.0 % in valdecoxib groups at 50～400 μmol·L-1.The proliferation index and the proportion of cell in the S and G2/M phase had the tendency to decrease,but there was statistical significance only in valdecoxib 400 μmom·L-1 group.③The expression of caspase-3 was increased.The expression of PCNA was decreased and the expression of COX-2 was not changed.CONCLUSION Valdecoxib inhibits clone 26 cell growth in a dose-and time-dependent fashion.The effect is mainly associated with apoptosis at low doses and also associated with cell cycle arrest at high doses.The apoptosis is associated with the increase of caspase-3.
关键词
戊地昔布 /
环氧化酶-2 /
小鼠肠癌clone26细胞 /
凋亡
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Key words
valdecoxib /
cyclooxygenase-2 /
mouse colorectal cancer clone 26 cell /
apoptosis
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李军霞;师晨霞;苏素文;张永健;王永利.
戊地昔布抑制clone 26肿瘤细胞增生及机制[J]. 中国药学杂志, 2007, 42(06): 441-444
LI Jun-xi;SHI Chen-xi;SU Su-wen;ZHNG Yong-jin;WNG Yong-li.
Inhibition of Valdecoxib on the Proliferation of Clone 26 Cancer Cell and Its Mechanism [J]. Chinese Pharmaceutical Journal, 2007, 42(06): 441-444
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脚注
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