Effect of Radix Astragali Gansuensis on Genetic Expression Spectra in Myocardium of Rats with Doxorubicin-Induced Cardiomyopathy
YU Jing1，2， SUO You-rui3， ZHANG Sheng-ping1，WANG Qiong-ying1， CHANG Peng1， WANG Han-qing2
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1. Department of Cardiology，Second Hospital of Lanzhou University， Lanzhou 730030， China；2.Researching and Developing Center of Natural Occurring Drugs， Lanzhou Institute of Chemical Physics， Chinese Academy of Sciences， Lanzhou 730000， China；3.Test and Measurement Center， Northwest Plateau Institute of Biology，Chinese Academy of Sciences，Xining 810001， China
OBJECTIVE To investigate the difference of genetic expression spectra between doxorubicin-induced cardiomyopathy in rats and normal controls by DNA microarray.To explore the effects of Radix Astragali Gansuensis on gene expression profiles in rats with doxorubicin-induced cardiomyopathy.METHODS Thirty Wistar rats were divided randomly into 3 groups,i.e control group2(n=10),doxorubicin group(n=10) and Radix Astragali+doxorubicin group(n=10).cDNA probes were prepared by labeling the mRNA extracted from myocardium and marked with Cy3-dTP and Cy5-dUTP respectively through reverse transcrition.DNA microarray were constructed by spotting PCR products of 4 000 and cDNAs onto specially treated glass slides and were then hybridized against the cDNA probes followed by fluorescent signals scanning.RESULTS Nine hundred and twenty three genes were detected with differential expressions.The expressions of 586 genes were found to be down-regulated and 337 ones up-regulated.Energy metabolism-related genetic expressions were down-regulated in tissues of rats with doxorubicin-induced cardiomyopathy compared with normal tissue.Expressions of apoptosis-related genes were up-regulated in tissues of doxorubicin-induced cardiomyopathy.Six up-regulated and 8 down-regulated genes in doxorubicin group were down-regulated and up-regulated in Radix Astragali+ doxorubicin group respectively.Expressions of 3 up-regulated genes in doxorubicin group were further enhanced in Radix Astragali+ doxorubicin group.CONCLUSION Apoptosis,energy metabolism disorder and immunity may participate the pathogenesis changes in rats with doxorubicin-induced cardiomyopathy.Radix Astragali Gansuensis can protect the heart in rats with doxorubicin-induced cardiomyopathy.Multiple gene expressions are involved to implement this pharmacological effect.
YU Jing;SUO You-rui;ZHNG Sheng-ping;WNG Qiong-ying;CHNG Peng;WNG Hn-qing.
Effect of Radix Astragali Gansuensis on Genetic Expression Spectra in Myocardium of Rats with Doxorubicin-Induced Cardiomyopathy [J]. Chinese Pharmaceutical Journal, 2007, 42(06): 437-440
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