Regulation of Aging-Related Genes by Chinese Traditional Medicine Bushen Yinao Pian Water Extract in SAMP10/Ta Mice Neural Cells
YANG Ting 1，2，4，ZHANG Chong1，2，HE Xin1，2，WANG Jin-gang3，LIU Gui-sheng1，CHEN Ging-xuan1
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1. Institute of Genetics and Developmental Biology，Chinese Academy，Sciences， Beijing 100080， China；2. Graduate School，Chinese Academy of Sciences， Beijing 100039， China；3.Beijing Kexin Bicheng Medical Technological Company Limited， Beijing 100086， China；4.China Patent Information Center，Beijing 100088，China
OBJECTIVE To investigate the anti-ageing molecular mechanism about Bushen Yinao Pian.METHODS The extract of Bushen Yinao Pian was added to the primary culture cells of SAMP10/Ta mouse brain.By using the technique of DDRT-PCR,medicine-specific expression genes were investigated.RESULTS Ten medicine-specific expression genes were found.Sequencing analysis showed that most of those fragments were homologous with some of certain gene cDNA that were related with senile.The fragments were homologous to the genes as follows: Mus musculus,clone IMAGE:4235872,mRNA;Mus musculus RIKEN cDNA A730024A03 gene(A730024A03Rik),mRNA;Mouse mRNA fragment for serum amyloid A(SAA) 3 protein;Heterogeneous Nuclear Ribonucleoprotein A0(HNRNP A0) homolog;Mus musculus OVCA1(Dph211) mRNA;proteasome(prosome,macropain) 26S subunit,non-ATPase,11;ATP-DEPENDENT MITOCHONDRIAL RNA HELICASE homolog;Mus musculus mitochondrial ribosomal protein S31(Mrps31),mRNA;Mouse CX43 gene;and tRNA-Ile.CONCLUSION Bushen Yinao Pian actually have anti-aging effects by affecting the expression-manners.
YNG Ting;ZHNG Chong;HE Xin;WNG Jin-gng;LIU Gui-sheng;CHEN Ging-xun.
Regulation of Aging-Related Genes by Chinese Traditional Medicine Bushen Yinao Pian Water Extract in SAMP10/Ta Mice Neural Cells [J]. Chinese Pharmaceutical Journal, 2007, 42(06): 427-431
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