摘要
目的研究咖啡因对大鼠工作记忆促进作用及其作用机制。方法大鼠经咖啡因及腺苷酸环化酶A1受体激动剂(CPA)、腺苷酸环化酶A2受体激动剂(CPCA)不同剂量给药后,采用三门行走迷路和水迷路实验对大鼠行为学进行评价。结果咖啡因能使大鼠在三门行走迷路和水迷路中的错误次数明显降低,在水迷路中潜伏期明显缩短,其中8,16 mg·kg-1的给药量组与空白对照组比较有显著性差异。腺苷酸环化酶A1受体激动剂0.30 mg·kg-1腹腔注射与咖啡因8 mg·kg-1灌胃同时给药时,咖啡因对大鼠工作记忆的促进作用被A1受体激动剂所拮抗;腺苷酸环化酶A2受体激动剂与咖啡因并用时,咖啡因对大鼠工作记忆的促进作用没有改变。结论咖啡因促进大鼠工作记忆的作用机制主要是阻断中枢腺苷酸环化酶A1受体,从而促进了中枢乙酰胆碱递质的释放。
Abstract
OBJECTIVE To study the effects of caffeine on improving the working memory and its mechanism.METHODS After administrations of the different doses of caffeine,N-6-cyclopentyladenosine(CPA) and 5-N-(cycloprodyl)-carboxmidoadenosine(CPCA),the ability of learning and memory in rats was tested by three-panel runway and water maze.RESULTS In three-panel runway and water maze,caffeine decreased the numbers of the errors,and latent period was shortened obviously in water maze.The dose of 8 and 16 mg·kg-1had very significant effect compared with control group.After CPA 0.3 mg·kg-1(ip) and caffeine 8 mg·kg-1(po) were administrated at the same time,the effect of caffeine on the working memory in rats was blocked by CPA.While CPCA and caffeine were used at the same dose,the effect of caffeine was unaffected.CONCLUSION The mechanism of caffeine improving the working memory in rats is related to blocking the adenosine A1 receptors of nerve centre and increasing the release of acetycholine in the nerve center.
关键词
咖啡因 /
腺苷酸环化酶A1受体激动剂 /
腺苷酸环化酶A2受体激动剂 /
三门行走迷路 /
水迷路
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Key words
caffeine /
N-6-cyclopentyladenosine /
5-N-(cycloprodyl)-carboxmidoadenosine /
three-panel runway /
water maze
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邵碧霞.
咖啡因对大鼠工作记忆促进作用及其机制的研究[J]. 中国药学杂志, 2006, 41(07): 512-514
SHO i-xi.
Effect and Mechanism of Caffeine on Improving the Working Memory in Rats [J]. Chinese Pharmaceutical Journal, 2006, 41(07): 512-514
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参考文献
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脚注
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