Effect of Ethanol and Aldehyde on Levels of Neurosteroids in Primary Cultural Astrocytes
ZHANG Cui-xin2，3， HOU Yan-ning1，2
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1.Bathune International Peace Hospital， Shijiazhuang 050082， China； 2. Hebei Medical University，Shijiazhuang 050017，China；3.Third Hospital of Hebei Medical University， Shijiazhuang 050051， China
OBJECTIVE To evaluate the effect of long term treatment of ethanol and aldehyde on levels of neurosteroids in primary cultural cortex astrocytes from fetal rats brain. METHODS The influence of ethanol and aldehyde on the livability and survival rate of primary cultural cortex astrocytes was evaluated by MTT.The concentration of unconjugated neurosteroids(PREG,DHEA and AP) and conjugated neurosteroids(DHEAS and PREGS)in Media from primary cultural cortex astrocyte were analyzed and determined using HPLC-MS/APCI.The effect on levels of neurosteroids after 72 h treatment with differents concentrations ethanol and aldehyde was evaluated. RESULTS No Significant effect of 12.5～100 mmol·L-1ethanol and 6.25～50 mmol·L-1aldehyde on the livability and survival rate of primary cultural cortex astrocytes was abserved.After 72 h treatment with three concentrations of ethanol and aldehyde,the levels of DHEAS after treatment with 25 mmol·L-1 ethanol and the level of DHEA after treatment with 50 mmol·L-1 ethanol were significant decreased(P<0.05).The level of AP was significant increased(P<0.05,P<0.001),but PREGS was significant decreased(P<0.05) after treatment with 50 mmol·L-1 or 100 mmol·L-1 ethanol.The level of PREG was significant decreased(P<0.05) after treatment with 100 mmol·L ethanol and 12.5 mmol·L-1 aldehyde,The level of DHEA was significant increased(P<0.05) after treatment with 25 and 50 mmol·L-1 aldehyde.Other treatment have no obviously effects on unconjugated and conjugated neurosteroids compared with PBS control group.CONCLUSION Both ethanol and aldehyde show effects on levels of neurosteroids in primary cultural cortex astrocytes with different characteristics.
ZHNG Cui-xin;HOU Yn-ning;.
Effect of Ethanol and Aldehyde on Levels of Neurosteroids in Primary Cultural Astrocytes [J]. Chinese Pharmaceutical Journal, 2006, 41(05): 395-398
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参考文献
[1] MORROW A L， JANIS G C，VANDOREN M J，et al. Neurosteroids mediate pharmacological effect of ethanol：A new mechanism of ethanol action[J] .Alcohol Clin Exp Res，1999，23，1933-1940.
[2] WEISS F， PORRINO L J. Behavioral neurobiology of alcohol addiction： recent advances and challenges [J] . J Neurosci，2002，22(9)：3332-3337.
[3] RUPPRECHT R. Neuroactive steroids： mechanisms of action and neuropsycho pharmacologicalproperties[J] . Psychoneuroendocrinology，2003，28(2)：139-168.
[4] ZWAIN I H，YEN S S C. Neurosteroidogenesis in astrocytes， oligodendrocytes and neurons of cerebral cortex of rat brain[J] . Endocrinology，1999，140（8）：3843-3852.
[5] CHOU R C，LANGAN T J. In vitro synchronization of mammalian astrocytic cultures by serum deprivation[J] . Brain Res Brain Res Protoc，2003，11(3)：162-167.
[6] REN J M，HOU Y N. Determination of unconjugated neurosteroids in rat brain regions by liquid chromatography-negative atmospheric pressure ionization mass spectrometry[J] . Acta Pharm Sin(药学学报)，2005，40(3)：262-266.
[7] REN J M，HOU Y N. Determination of conjugated neurosteroids in rat brain regions by liquid chromatography-negative atmospheric pressure ionization mass spectrometry[J] . Chromatography，2004，22(6)：575-578.
[8] KAWATO S，YAMADAB M，KIMOTO T. Brain neurosteroids are 4th generation neuromessengers in the brain：Cell biophysical analysis of steroi signal transduction[J] . Adv Biophys，2001， 37：1-30.
[9] MAJCHROWICZ E. Induction of physical dependence upon ethanol and the associated behavioral changes in rats[J] . Psychopharmacologia，1975， 43(3)：245-254.
[10] FRYE G D，CHAPIN R E，VOGEL R A，et al. Effectsof acute and chronic 1，3-butanediol treatment on central nervous system function：a comparison with ethanol[J] . J Pharmacol Exp Ther，1981，216(2)：306-314.
[11] CARBONI E， WIELAND S， LAN N C， et al. Anxiolytic properties of endogenously occurring pregnanediols in two rodent models of anxiety[J] . Psychopharmacology，1996，126(2)：173-178.
[12] BROT M D，AKWA Y，PURDY R H，et al. The anxiolytic-like effects of the neurosteroid allopregnanolone： interactions with GABA(A) receptors[J] . Eur J Pharmacol，1997，325(1)：1-7.
[13] MELCHIOR C L， RITZMANN R F. Dehydroepiandrosterone is an anxiolytic in mice on the plus maze[J] . Pharmacol Biochem Behav，1994，47(3)：437-441.
[14] REDDY D S， KULKARNI S K. The role of GABA-A and mitochondrial diazepam-binding inhibitor receptors on the effects of neurosteroids on food intake in mice[J] . Psychopharmacology，1998，137(4)：391-400.
[15] MELCHIOR C L， RITZMANN R F. Pregnenolone and pregnenolone sulfate， alone and with ethanol， in mice on the plus-maze[J] . Pharmacol Biochem Behav，1994，48(4)：893-897.
[16] PURDY R H， MOORE PH J R， MORROW A L， et al. Neurosteroids and GABAA receptor function[J] . Adv Biochem Psychopharmacol，1992，47：87-92.
[17] SANNA E， TALANI G， BUSONERO F， et al. Brain steroidogenesis mediates ethanol modulation of GABAA receptor activity in rat hippocampus[J] . J Neurosci，2004， 24(29)：6521-6530.
[18] FAINGOLD C L，N'GOUEMO P，RIAZ A.Ethanol and neurotran smitter interactions-from molecular to integrative effects.Prog Neurobiol,1998，55（N5）: 509-535.
