OBJECTIVE To investigate the effects of chitosan gel and chiston /gelatin mixed film on preventing peritoneal adhesion in rats. METHODS 240 rats were divided into jumpbogroup A(144 rats,with chitosan gel) and jumpbogroup B(96 rats,with chiston /gelatin mixed film).Jumpbogroup A were further divided into 3 groups: the peritoneal adhesion were induced with injury(group A1)、Talc Powder(A2) and blood vessel Ligation(A3) respectively.Each group were randomly divided into control group and experimental group further.In the experimental group,chitosan gel were spreaded over the serosal surface of the treated procussus vermiformis.Jumpbogroup B were divided into 4 groups further: while the wound surface of procussus vermiformis in group B2,B3 and B4 were coverd with pure chitosan film、chiston film containing 10% gelatin and chiston film containing 50% gelatin respectively,no film was used in group B1 as control group.2 and 4 weeks after surgery,the peritoneal adhesion degree were evaluated with naked eye after celiotomy,and the adhesive blind-ending vermiform processes tissues were histopathologically observed.RESULTS In jumpbogroup A:① peritoneal adhesion evaluation: 2 and 4 weeks after surgery,the experimental group in group A1 and A3 had milder adhesions than that in the control group.But in group A2,there was no significant difference between the control and experimental group.②pathological change: group A1,A3 was mainly with fibroplasia,while massive foreign-body giant cell reaction(FBGCR) and granuloma fomation were observed in group A2.In jumpbogroup B:① degradation speed of film: group B3 had a faster degradation than group B2 but slower than group B4.②2 and 4 weeks after surgery,adhesions in Group B1 was milder than any other experimtenal groups treated with various films;2 weeks after surgery,the adhesions in group B2 was significantly milder than that in group B3 and B4;4weeks after surgery,there were no significant difference of adhesions level among group B2,B3 and B4;③Histopathological examinaiton indicated that inflammatory cell infiltration and fibroplastic proliferation dominated in local lesion,and FBGCR existed in all groups.CONCLUSION Chitosan gel has perfect effect in protecting peritoneal adhesion induced by traumatic or ischemic;Pure chitosan film could exacerbate peritoneal adhesion and chitosan containing gelatin could exacerbate peritoneal adhesion further.
ZHNG Zhi-ling;ZHOU Xie-li.
Effects of Chitosan on Preventing Peritoneal Adhesion in Rats [J]. Chinese Pharmaceutical Journal, 2006, 41(05): 343-346
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