目的建立RP-HPLC测定不同种族人尿中右美沙芬及去甲右美沙芬含量的方法。方法尿样经酶水解，酸、碱提取纯化后，20μL直接进样于HPLC系统，经苯基柱分离后荧光(激发波长280 nm，发射波长310 nm)检测，流动相为乙腈10 mmol·L^-1KH₂PO₄，10 mmol·L^-11-己烷磺酸钠缓冲溶液(51∶49，pH 4.0)，柱温为25℃。结果样品中右美沙芬及去甲右美沙芬得到较好分离，线性范围右美沙芬在0.02～2 μg·mL^-1,r=0.999 8，n=9，最低检测限为0.4 ng·mL^-1。去甲右美沙芬0.5～30 μg·mL^-1，r=0.998 5，n=7，最低检测限为0.8 ng·mL^-1。结论该方法简便可靠,适用于不同种族人尿中右美沙芬及去甲右美沙芬含量测定和多态性的表型研究。

OBJECTIVE To extablish a reverse phase HPLC method for the determination of dextromethorphan and dextrophan in human urine in different races. METHODS The urine samples were hydrolyzed by enzyme,distilled and purified by acid and alkali.Then 20μL extracted sample was directly injected into the HPLC system with Phenyl column,and fluorescence detection (ER at 280 nm,EX at 310 nm).Acetonitrile water containing 10 mmol·L^-1KH₂PO₄and 10 mmol·L^-11-hexane sulfonate-Na (51∶49，pH4.0) was used as the mobile phase.The column temperature was 25℃. RESULTS The dextromethorphan and dextrophan were seperated satisfactorily.The linear ranges of dextromethorphan and dextrophan were 0.02～2 μg·mL^-1(r=0.999 8,n=9) and 0.5～30 μg·mL^-1,respectivly.The detection limits were 0.4 and 0.8 ngl·mL^-1,respectivily. CONCLUSION The method is simple and reliable.The results shows that RP-HPLC is a good method for measuring both dextromethorphan and dextrophan in human urine in different races and for studying the phenotype of the genetic polymorphisms.