目的对丹参水溶液提取物总丹酚酸和银杏叶提取物EGb761对血管平滑肌细胞损伤的保护作用进行了比较研究。方法选用培养的10～20代大鼠血管平滑肌细胞用于实验研究。细胞用H2O22(100μmol·L-1)处理1h造成细胞氧化损伤模型，采用MTT方法观察总丹酚酸和EGb761对H2O2损伤细胞生存率的影响，同时以乳酸脱氢酶(LDH)、丙二醛(MDA)、一氧化氮(NO)3种物质变化作为指标，观察了总丹酚酸和EGb761对H2O2损伤血管平滑肌细胞的保护作用。结果总丹酚酸100，10 μg·mL-1能显著提高细胞的存活率，而总丹酚酸1μg·mL-1和EGb761 100，10，1μg·mL-1对细胞的存活率没有明显的升高作用；总丹酚酸100 μg·mL-1，EGb761 100，10，1 μg·mL-1均能显著降低H2O2损伤细胞上清液中LDH 的量，总丹酚酸10，1 μg·mL-1作用不显著；EGb761 100，10 μg·mL-1能明显降低细胞上清中MDA的量，EGb761 1 μg·mL-1及总丹酚酸100，10，1μg·mL-1作用不明显；总丹酚酸100，10，1 μg·mL-1和EGb761 100，10 μg·mL-1明显降低H2O2损伤引起NO的释放。结论总丹酚酸和EGb761对H2O2损伤的血管平滑肌有保护作用，但作用机制似乎有所不同，需要做进一步研究。
Abstract
OBJECTIVE To study and compare the protective effects of salvianolic acids and EGb761 on the cultured rat vascular smooth muscle cells (VSMC). METHODS The injuries of VSMC induced by hydrogen peroxide (100 μmol·L-1) were indicated as cell viability,release of lactic dehydrogenase (LDH) and nitride oxide (NO) and the production of malondildehyde (MDA) in medium. RESULTS Salvianolic acids 100 and 10 μg·mL-1significantly increased viability of VSMC reduced byH2O2.Salvianolic acids 100 μg·mL-1and EGb761 100，10 and 1 μg·mL-1significantly decreased the release of LDH in supernatant.EGb761 100 and 10 μg·mL-1apparently decreased MDA in supernatant.Salvianolic acids 100,10 and 1 μg·mL-1and EGb761 100 and 10 μg·mL-1also decreased the release of NO caused byH2O2. CONCLUSION Salvianolic acids and EGb761 had protective effects on VSMC,but the mechanisms remains to be further studied.
关键词
血管平滑肌细胞 /
H2O2 /
丹参(总丹酚酸) /
银杏叶提取物(EGb761)
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Key words
vascular smooth muscle cells /
hydrogen peroxide /
Radix Salviae Miltiorrhizae(salvianolic acids) /
extract of Ginkgo biloba (EGb761)
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参考文献
[1]Touyz RM.Molecular and cellular mechanisms regulating vascular function and structure-implications in the pathogenesis of hypertension[J]. Can J Cardiol, 2000，16(9)：1137.
[2]Zhang M,Zhang B H,Chen L, et al. Overexpression of heme oxygenase-1 protects smooth muscle cells against oxidative injury and inhibits cell proliferation[J]. Cell Research, 2002，12(2)：123.
[3]王 洁,张均田.总丹酚酸抗脑缺血与抗血栓的关系[J].中国药理学通报，1999，15(3)：237.
[4]任德成,杜冠华,张均田.总丹酚酸对脑缺血再灌注损伤的保护作用[J].中国药理学通报，2002，18(3)：275.
[5]Chen X,Chen W Z.Recent pharmacological progress of Ginkgo biloba extract for cardiovascular and neuronal diseases[J]. Chin J Int Med, 1996，2：300.
[6]Campos-Toimil M,Lugnier C,Dror-Lefaix MT, et al. Inhibition of Type 4 phosphodiesterase by rolipram and Ginkgo biloba extract (EGb 761) decreases agonist-induced rises in internal calcium in human endothelial cells[J]. Arterioscler Thromb Vasc Biol, 2000，20：E34.
[7]徐叔云,卞如濂,陈修.药理实验方法学[M].第2版.北京：人民卫生出版社，1991：535.
[8]张均田.现代药理实验方法[M].北京：北京医科大学中国协和医科大学联合出版社，1998：1226，1317.
[9]Sakurai H,Kohsaka H,Lui MF, et al. Nitric oxide production and inducible nitric oxide synthase espression in inflammatory arthritides[J]. J Clin Invest, 1995，96：2357.
[10]Kaikobad I.Oxidant signaling in vascular cell growth,death,and survival[J]. Circ Res, 2000，87：179.
[11]Coassin M,Maiorino M,Zamburlini A, et al. Effect of hydrogen peroxide on calcium homeostasis in smooth muscle cells[J]. Arch Biochem Biophis, 1992，297：265.
[12]Berry C,Brosnan MJ,Fennell J, et al. Oxidative stress and vascular damage in hypertension[J]. Curr Opin Nephrol Hypertension, 2001，10：247.
[13]Sharpe PC,Liu WH,Yue KKM, et al. Glucose-induced oxidative stress in vascular contractile cells[J]. Diabetes, 1998，47：801.
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脚注
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基金
自然科学基金项目(No.3001161940;30171148);国家高技术研究发展计划(863)项目(No.2001AA234071)
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