人GPx1基因的克隆及其序列分析

贺华君;范立强;袁勤生;谢倩;吴祥甫

中国药学杂志 ›› 2001, Vol. 36 ›› Issue (05) : 343-345.

中国药学杂志 ›› 2001, Vol. 36 ›› Issue (05) : 343-345.
生物技术

人GPx1基因的克隆及其序列分析

  • 贺华君;范立强;袁勤生;谢倩;吴祥甫
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Cloning and sequence analysis of human glutathione peroxidase 1

  • 1.华东理工大学生物反应器工程国家重点实验室,生物化学研究所,上海 200237;2.中国科学院上海生物化学研究所,上海 200031
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摘要

目的克隆中国人谷胱甘肽过氧化物酶(GPx1)的cDNA。方法用逆转录聚合酶链反应(RT-PCR),以中国人胎盘组织总RNA为模板,扩增中国人谷胱甘肽过氧化物酶(GPx1)的cDNA,进行序列分析。计算机分析其二级结构并比较已发现的人GPx家族的5种GPx氨基酸序列。结果从中国人胎盘组织中克隆的GPx1基因,与国外文献报道相比,只有1个氨基酸残基发生变异,即leu22变为Val22,该变化不影响GPx1活性中心的结构;人GPx的硒结合区及活性中心区域的氨基酸序列是高度保守的。结论首次克隆了中国人体特异的胞质内GPx1基因,为其生物学活性及结构与功能的关系的研究创造了条件。

Abstract

OBJECTIVE To clone the cDNA of human glutathione peroxidase 1(GPx1).METHODS The total RNA was isolated from normal Chinese human placenta and was amplified as the template by RT-PCR.It was inserted into pSK plasmid to analyze its sequence;the sequences of 5 known GPx gene were compared. RESULTS The GPx1 cDNA from placenta had 1 base pare and 1 amino acid residue (Leu22mutated to Val22),different from that of reported.But it didn't alter the secondly structure of GPx selenocysteint residue and the active sites were highly conserved in 5 GPxs. CONCLUSION The human GPx1 gene of Chinese was cloned for the first time.

关键词

谷胱甘肽氧化物酶 / 基因克隆 / 序列分析 / GPx家族 / 超氧化物歧化酶

Key words

glutathione peroxidase / gene cloning / sequence analysis

引用本文

导出引用
贺华君;范立强;袁勤生;谢倩;吴祥甫. 人GPx1基因的克隆及其序列分析[J]. 中国药学杂志, 2001, 36(05): 343-345
.华东理工大学生物反应器工程国家重点实验室;生物化学研究所;上海 00;.中国科学院上海生物化学研究所;上海 000. Cloning and sequence analysis of human glutathione peroxidase 1[J]. Chinese Pharmaceutical Journal, 2001, 36(05): 343-345

参考文献

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