目的 建立一种准确、快速、简便的方法测定胰岛素注射液的含量。方法 采用毛细管区带电泳法(CZE)以pH 7.8，0.05 mol·L^-1的磷酸盐缓冲液做运行液，进行胰岛素注射液中各组分的分离，工作电压18 kV(运行电流130～140 μA)，以波长214 nm紫外检测装置进行检测，并以苯巴比妥作内标，测定胰岛素含量。结果 在此条件下，胰岛素注射液中各成分可以完全分离，保留时间为4～8 min，胰岛素的平均出峰时间为4.973 min(RSD0.66%，n=10)，内标为5.621 min(RSD0.64%,n=10)。胰岛素浓度在10～100 u·ml^-1范围内，浓度与胰岛素峰面积与内标峰面积比值之间呈良好的线性关系。平均回收率98.95%(RSD0.97%,n=6),3批样品日内测定8次，RSD＜1.5%，日间测定3次 RSD＜1.0%，两批样品与HPLC测定值比较最大相差0.52%。结论 在本实验条件下应用CZE测定胰岛素注射液的含量可以收到满意的结果，与HPLC相比有相同的准确性，但更为快速、简便、节省溶剂。

OBJECTIVE To study an accurate,simple and rapid method with capillary zone clectrophoresis (CZE) for the determination of Insulin injections.METHODS In CZE analysis,pH 7.8 0.05 mol·L^-1running phosphate buffer,a constant voltage 18 kV (current of 130～140 μA) and wave length 214 nm UV detector were used.Phenylbarbital was used as the internal standard.RESULTS In this experimental condition,the components in insulin injection were separated completely,the retention times of insulin and the internal standard were 4.973 min (RSD 0.66% n=10) and 5.621 min (RSD 0.64% n=10) respectively.A good linearity was obtained between concentration and ratio of peak areas for insulin and the internal standard over the con range of 10～100 u·ml^-1.The recovery was 98.95% (RSD 0.97% n=6) and RSDof three samples determined in a day and day to day were ＜1.5%(n=8) and ＜1.0% (n=3) respectively.CONCLUSION The proposed CZE method can be used for the determination of Insulin injection with satisfactory results and has same accuracy as HPLC,but more rapid and solvent-saving.