目的 测定胸腺肽溶液中α₁的含量。方法 以胸腺肽α₁纯品作为标准品，用高效毛细管电泳测定，未涂层石英毛细管柱(78 cm×30 μm)；电泳缓冲溶液为0.05 mol·L^-1磷酸盐缓冲液(pH 7.0)；运行电压15 kV；负压进样(66.7 kPa)；温度25℃；检测波长200 nm；进样时间1 s。用比例色谱的方法确定胸腺肽溶液中α₁峰的纯度。结果 胸腺肽α₁标准溶液的线性范围50～800 μg·ml^-1，低、中、高3种浓度的平均回收率分别为87.2%，91.0%，97.4%。分离出的胸腺肽溶液中α₁的组分峰为单一组分峰，3批胸腺肽溶液中α₁的质量百分比分别为1.24%，0.812%，0.732%。结论 该方法可用于胸腺肽质控中α₁含量的测定。

OBJECTIVE To determine the content of thymosin alphal 1 in thymopeptide.METHODS High Performance Capillary electrophoresis (HPCE) analysis method was used at 25℃,using thymosin alphal 1 as the standard reference.The separation was performed on a 78 cm×30 μm uncoated sillica capillary column with the voltage 15 kV.The electrophoresis solution was 0.05 mol·L^-1phosphate buffer (pH 7.0).The sample was detected with a UV-detector at 200 nm.RESULTS The linearity was obtained over the range of 50～80 μg·ml^-1.The average recoveries of the check sample at the low,middle and high concentrations were 87%,91% and 97%,respectively.The mass content of thymosin alpha1 in 3 lots of thymopeptide solution were 1.24%,0.812% and 0.732% respectively.CONCLUSIONS The method could be used to determine the content of thymosin α-1 in thymopeptide for quality control.