目的 从基因表达水平研究高山红景天苷对大鼠心脏内分泌功能的影响。方法 分别给各组大鼠按15 mg·kg-1·d-1ip红景天苷氯化钠溶液。饲养30 d后，断头处死。冷酚法提取心房总RNA，用(α32P)标记的大鼠心钠素前体原cDNA控针与各组大鼠心房总RNA做Northern blot杂交和Dot blot杂交，以检测心房ANP-mRNA的含量。结果 老年大鼠ANP-mRNA含量明显低于青年大鼠，注射红景天苷后，老年大鼠ANP-mRNA含量增加而青年大鼠减少。结论 老化过程中ANP基因表达能力明显衰退，红景天具有一定的抑制心脏内分泌功能老化的作用。
Abstract
OBJECTIVE To study the effects of gadoloside on atrial natriuretic peptide (ANP) gene expression.METHODS The rats were abdominally injected with sodium chloride solution of gadoloside 15 mg·kg-1·d-1for 30 days.Atria total RNA was extracted by the cold phenol method.The ANP-mRNA contents were determined using the Northem blot and Dot blot hybridization technique with α-32P-Labelled r-prepro-ANP-cDNA probe.RESULTS The ANP-mRNA contents of old rats were remarkably less than that of young rats.Salidroside increased the ANP-mRNA content of old rats,whereas it decreased the ANP-mRNA content of young rats.CONCLUSION The ANP gene expression declined during aging development and salidroside possessed anti-aging effects in the heart endocrine function.
关键词
高山红景天苷 /
心钠素 /
基因表达
{{custom_keyword}} /
Key words
gadoloside /
ANP /
gene expression
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］汤键.分子杂交技术在肽类内分泌研究中的应用［C］.唐山：全国第二届心肺内分泌学术会议论文摘要，1988：10.
［2］Nakayama K,Ohkubo H,Hirose T,et al.mRNA Sequence for human cardiodilatin ANF precursor and regulation of precursor mRNA in rat atria［J］.Nanure,1984,310:699.
［3］Tawaragi Y,Fuchimura K,Tanaka S,et al.Gene and precurser struc-ture of human C-tyge natriuretic peptide［J］.Bichem Biophy Res Com-mun,1991,175(2):645.
［4］De Bold AJ,Flynn TG.Cardionatrin I-a novel heart peptide with po-tent diuretic and natriuretic properties［J］.Life Sci,1983,33(3):297.
［5］洪敏，周晓萍，刘剑凯.冷酚法提取RNA及质量鉴定［J］.白求恩医科大学学报，1992，18(2)：105.
［6］J.萨母布鲁克，E.F.费里奇，T.曼尼阿蒂斯.分子克隆实验指南［M］.第2版，北京：科学出版社，1995：363.
［7］J.萨母布鲁克，E.F.费里奇，T.漫尼阿蒂斯.分子克隆实验指南［M］.第2版，北京：科学出版社，1995：372.
［8］洪敏，Barden N.体外转录系统质粒pNI11和pNI12的构建［J］.白求恩医科大学学报，1989，15(5)：433.
［9］Seidman CE,Duby AD,Choi E,et al.The structure of rat preproatrial natriuretic factor as defined by a complementary DNA clone［J］.Sci-ence,1984,225:234.
［10］Wu JP,Deschepper CF,Gardner DG.Perinatal expression of the atrial natriuretic factor gene in rat cardiac trissue［J］.Am J Physiol,1988,255(3 pt 1):E388.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
国家自然科学基金资助项目(39270732)
{{custom_fund}}
