摘要
目的:采用高效液相色谱法定量测定MS-551(MS)的血浆浓度?方法:应用国产ZY1104型C18柱,以甲醇-0.1mol·L-1醋酸铵(60∶40)为流动相,检测波长270nm?结果:经外标法测定,MS血浆浓度在0.1～2.0μg·ml-1呈良好线性(r=0.9990),检出限为0.85ng,同时观察了麻醉犬用药后MS血浆浓度与心电图QTc的关系?结论:该方法简便、实用、准确?
Abstract
OBJECTIVE:To report a method for the determination of MS-551(MS) concentrations in plasma by HPLC.METHODS:MS was separated on a C18 column(5μm,4.6mm×15cm) by using methanol -0.1M ammonium acetate (60∶40) as mobile phase with detection wavelength at 270nm.Quantification was carried out based on the external standard of MS.RESULTS:There was a good linearity over the range from 0.1 to 2.0μg·ml-1 of MS,Y=8.6963×10-2+3.55×10-6X(r=0.9990).The average recovery was 94.27%.The detection limit was 0.85ng.At the same time,the relationship between the plasma concentrations of MS and QTc was determined in anaesthetized dogs with MS.CONCLUSION:This method is simple,practical and accurate.
关键词
MS-551 /
高效液相色谱法 /
血浆浓度
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Key words
MS-551 /
HPLC /
plasma concentration
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孟玲;李小鹰;廖杰.
用高效液相色谱法测定MS-551的血浆浓度[J]. 中国药学杂志, 1999, 34(11): 759-761
Meng Ling .
Determination of MS-551 concentration in plasma by HPLC[J]. Chinese Pharmaceutical Journal, 1999, 34(11): 759-761
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参考文献
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2 Kamiya J，Ishii M，Katakami T．Antiarrhythmic effects of MS551，a new class Ⅲ antiarrhythrnic gaent，on canine models of ventricular arrhythmia．J pn J Pharmacol．1992，58(2)：112．
3 Kondoh K，Hashimoto H，Nishiyama H，et al．Effects of MS-551，a new class Ⅲ antiarrhythmic drug，on programmed stimulation-induced ventricular arrhythmias，electropysiology，and hemodynamics in a canine myocardial infarction model．J Cardiovasc Pharmacol，1994，23(4)：675．
4 Kamiya J，Ishii M，Yoshihara K，et al．MS-551：Pharmaoological profile of a novel class Ⅲ antiarrhythmie gaent．Drug Dev Res，1993，30(1)：39．
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脚注
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