高效毛细管电泳研究氯胺酮对映体血药浓度测定方法

程庆春;李学仁;胡汉芳;王洪;丁天惠

中国药学杂志 ›› 1999, Vol. 34 ›› Issue (03) : 189-191.

中国药学杂志 ›› 1999, Vol. 34 ›› Issue (03) : 189-191.
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高效毛细管电泳研究氯胺酮对映体血药浓度测定方法

  • 程庆春;李学仁;胡汉芳;王洪;丁天惠
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Study on the determination of ketamine enantiomers in plasma by high-performance capillary electrophoresis

  • Cheng Qingchun
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摘要

目的:建立高效毛细管电泳测定氯胺酮对映体血药浓度的方法。方法:血浆样品用三氯醋酸沉淀蛋白质,缓冲溶液为10mmol·L-1羧甲基-β-CD(CM-β-CD)加75mmol·L-1磷酸盐缓冲溶液,pH=5.00,紫外检测λ=210nm。结果:线性范围为2.5~25.0μg·ml-1,平均回收率为101.8%,日内和日间误差分别小于3%和5%。结论:本方法分析速度快,手性分离效率高,可用于开展临床研究。

Abstract

OBJECTIVE:To develep HPCE method for the determination of ketamine enatiomers in human plasma.METHODS:Plasma samples were handled by the addition of trichloroacetic acid to precipitate protein.The buffer was CM-β-CD-phosphate 7.5 mmol·L-1~75mmol·L-1with pH5.0.The detection wavelength was 210nm.RESULTS:The method was linear over the range of 2.5~25.0μg·ml-1.The average recovery was 101.8% and the validation of inter-day was less than 3% and 5%,respectively.CONCLUSION:This method is simple and rapid.The resolution of ketamine enantiomers is satisfied.It provides a suitable assay for clinical study.

关键词

高效毛细管电泳 / 氯胺酮对映体 / 血药浓度

Key words

HPCE / ketamine enantionmers

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导出引用
程庆春;李学仁;胡汉芳;王洪;丁天惠. 高效毛细管电泳研究氯胺酮对映体血药浓度测定方法[J]. 中国药学杂志, 1999, 34(03): 189-191
Cheng Qingchun. Study on the determination of ketamine enantiomers in plasma by high-performance capillary electrophoresis[J]. Chinese Pharmaceutical Journal, 1999, 34(03): 189-191

参考文献

1 蒋光祖.手性药物的研究与开发.中国药学杂志,1996,31(10):579. 2 White PF,Ham J,Way WL,et al.Pharmacology of ketamine isomers in surgical patients.Anesthesiology,1980,52:231. 3 叶平安,朱银南著.实用麻醉药理学.西安:陕西科学技术出版社,1995:348. 4 ChangT,Glazko AJ.A gas chromatographic assayfor ketamine in human plasma.Anesthesiology,1972,36:401.

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