目的:检测培养的大鼠肾上腺髓质嗜铬细胞分泌的儿茶酚胺。方法:用原代培养的大鼠肾上腺髓质嗜铬细胞,建立了培养细胞分泌的儿荼酚胺(去甲肾上腺素,肾上腺素和多巴胺)的高效液相色谱-电化学检测法。结果:在静息状态下,细胞在20min内分泌的儿茶酚胺为每106个细胞(73.29±15.32)ng,用乙酰胆碱等药物刺激后分泌明显增加?结论:用该方法可以灵敏地检出培养的肾上腺髓质嗜铬细胞分泌的儿茶酚胺,细胞基础分泌稳定,对药物刺激反应良好>目的:检测培养的大鼠肾上腺髓质嗜铬细胞分泌的儿茶酚胺?方法:用原代培养的大鼠肾上腺髓质嗜铬细胞,建立了培养细胞分泌的儿荼酚胺(去甲肾上腺素,肾上腺素和多巴胺)的高效液相色谱-电化学检测法。结果:在静息状态下,细胞在20min内分泌的儿茶酚胺为每106个细胞(73.29±15.32)ng,用乙酰胆碱等药物刺激后分泌明显增加?结论:用该方法可以灵敏地检出培养的肾上腺髓质嗜铬细胞分泌的儿茶酚胺,细胞基础分泌稳定,对药物刺激反应良好。
Abstract
OBJECTIVE:To study the secretion of catecholamine primary cultures of chromaffin cells from rat adrenal medulla were used.METHOD:Catechoalmines(norepinephrine,epinephrine and dopamine) were measured by high-performance liquid chromatography-electrochemical detection technique.RESULTS:Catecholamine released by chromaffin cells with in 20min without slimalus was (73.29±15.32) ng/106cells.When acetylcholine,nicotine or muscarine was added,the secretion of catecholamine was then increased.CONCLUSION:Using high-performance liquid chromatography-electrochemical detection technique,we can detect sensitively catecholamine released by cultured rat chromaffin cells.
关键词
肾上腺髓质嗜铬细胞 /
儿茶酚胺 /
高效液相色谱-电化学检测法
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Key words
adrenal medullary chromaffin cells /
catecholamine /
high performance liquid chromatography-electrochemical detection technique
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参考文献
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