目的:探索准确而简便地测定盐酸洛美沙星葡萄糖注射液的含量。方法:采用高效液相色谱法，用硅胶柱，以十二烷基硫酸钠磷酸二氢钾混合溶液-甲醇(60:40)为流动相，在波长287 nm处进行检测。结果:在5. 55 ～111.00μg·ml^-1的浓度范围具有良好的线性关系，r = 0.999 8，平均回收率99.59%(n=5),RSD=0.54%。结论:本法适用于成品检验及杂质或降解产物的检查。

OBJECTIVE: To find a precise, simple and convenient method for concentration determination of lomefloxacin hydrochloride and glucose injection. METHOD: A HPLC method was chromatographed on silica columns with a mobile phase of a mixture solution of sodium dodecyl sulphate and potassium dihydrogen phosphate-methanol(60:40) with detection at wave-length 287 nm. RESULTS: The linear range was 5.55～111. 00μg·ml-1，the average recovery 99. 59%，and RSD 0.54%(n=5).CONCLUSION:The method can be used to determine finished product and impurity.