在囯内首先建立了大肠杆菌DNA修复试验（E,coli recA/uvrB differential DNA repair test,E. coli DDRT)，用21种各类化合物进行方法学验证。结果提示，与Ames 试验的符合率为65/%(13/20)，与丈献报道E.coli DDRT(其它菌株）试验结果的符合率为80%(12/15〉，与幼物致癌试验结果的符合牟为76.5%(13/17)，其中E.coli DDRT试验结果阳性而Ames城验阴性的有4-Aminophenol.Catechol，DES，Thioacetamide；而E.coli DDRT试验结果阴性，Ames试验阳性的有4-AAF,Benzidine，CP，DMSO。基于这些结果，可以认为本实验室建立的大肠杆菌recA/uvrB DNA修复试验方法具有实用价值和重现性，可进一步建立该菌株的体内试验方法。
Abstract
The E. coli recA/uvrB differential DNA repair test(E.coli DDRT) was first established and validated with 21 various compounds in China. The results showed that 13 were positive among 21 chemicals tested. The general concordance was 65% (13/20) with Ames test,80% (12/15) with E.coli DDRT(using different strains) results published in the literature,and 76. 5%(13/17) with animal carcinogenecity test. Among these compounds,4-aminophenol,catechol ,DES,thioacetamide were positive in E.coli DDRT but negetive in Ames test ,and conversely,4-AAF,benzidine ,CP,DMSO were negetive in E.coli DDRT but positive in Ames test.The reasons for this disagreement were discussed. The differential killing index (DKI) was caculated to quantitatively compare relative genotoxic potency among chemicals tested. These results suggest that the method established by now is practical and reproducible and can be applied to screen the mutageneeity of chemicals.
关键词
大肠杆菌K-12 /
DNA修复 /
致突变试验
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Key words
E. colik-12. DNA repair /
mutageneeity test
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参考文献
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