以甲醇-水（60:40)为流动桕，在Spheri-5,RP-18，5micron(220mm×4.6mm,美国家SP公司）柱上，研究建立了测定蟾酥及其制剂中蟾毒内酯含量的RP-HPLC。流速为1ml/min，检测波长299nm,外标法。浓度在19.3～96.5μg/ml范围内线性关糸良好，r=0.9996。本方法日内误差和日间误差分别平均为1.26%和4.66%。该方法简便、快速、精确。

A RP-HPLC method was established for the determination of bufogenin in Bufo venon(Chansu) and its preparations. The mobile phase consisted of a mixture of CH₃OH-H₂O(60:40), The flow rate was 1 ml/min. The detection was at 299 nm and no internal standard was used. Linear calibration curve for bufogenin was measured with a rang oi 19. 3 to 96. 5 μg/ml. With the correlation coefficient of 0.9996,the RSD of within-day and day to day was 1.26% and 4. 66% respectively.The method is simple,fast and accurate.