以不同pH位的甲醇一水体系组成40多种流动相，在10多种不同牌号的ODS色谱柱上，对 200种药物进行了RP-HPLC色谱行为测试．并以保留位为流动相pH值的函数特征作指标，将药物分 成碱性、酸性和中性3类。在微机上分别采用Foxbase+2.1和Foxpro 2. 5 for windows语言编写数据 库程序．记录药物色谱峰的保留时间和半峰宽等参数。提出了“pH4 - 5碱性药峰序变动区”新概念。药物 色谱行为分类和数据库的建立有利于从宏观上把握药物的色谱行为趋势，减少不必要的实验条件和实 验次数，对内标物的选择及分离度的改善和保留值的增减趋势均可作出事先的判断。将色谱数据库与实 际应用相结合，取得了较好的效果。

The chromatographic behaviours of about two hundred of drugs were investigated under reversed-phase condition using methanoI-water system of more than 40 different pH values as elucnts. The capacity factors of these drugs were measured on several ODS columns. In terms of the characterization of retention values as function of the mobile phase pH ?the drugs were divided into three classes ： basic.acidic and neutral. The elution order reversal was frequently encountered with basic drugs within pH4～5. The database program was written by using the languages of Foxbnse +2.1 and of Foxpro 2.5 for windows.The databases and the classification might play important roles in the recognition of the trends of chromatographic behaviour of these drugs and the determination of initial chromatographic condition and reduce analysis time and efforts.It may help us to obtain the optical separation results ano to select the best internal standards.