Abstract: Objective To explore the mechanism of mesenteric lymph duct ligation against hepatic injury in rats by two-hit of hemorrhage and LPS. Methods 45 Wistar rats were divided into three groups: the ligation group, the non-ligation group and sham group, and the two-hit model was established by hemorrhage and LPS, mesenteric lymph was blocked by ligating mesenteric lymph duct in ligation group. After 24 hours of operation trauma, taken out the liver making for pathological section, and the hepatocellular apoptosis rate was determined by method of TUNEL, the expression of BCL-2 and BAX protein were determined by immunohistochemical test. At the same time, taken out liver making for homogenate of 10 percent, the activity of MPO and ATPase and the contents of TNFα and IL-6 were determined in hepatic homogenate. Results After two-hit, the hepatocellular apoptosis rate and expression of BAX protein in non-ligation group were significantly increased compared with sham group and ligation group, and expression of BCL-2 protein was significantly lower respectively. The contents of MPO, TNFα and IL-6 in hepatic homogenate of non-ligation group were significantly increased than that of sham group, and the activity of ATPase in hepatic homogenate was significantly lower. But the ATPase in hepatic homogenate of ligation group were significantly increased than that of non-ligation group, and the MPO, TNFα and IL-6 in hepatic homogenate of ligation group were significantly lower compared with non-ligation group. Conclusion The results demonstrated that the mechanism of mesenteric lymph duct ligation lightens the hepatic injury of rats was relating to the mesenteric lymph blockage reduces the TNFα and IL-6 and improves the expression of BCL-2 protein and the activity of ATPase in liver.

Key words: Mesenteric lymph duct, Ligation, Liver injury, Two-hit, Inflammatory mediators, Apoptosis