Abstract: The repair of massive bone defect is one of the troubling problems in clinic. The therapeutic efficacy treated by traditional methods, such as repair with autograft, alograft and biomaterial-filling and so on, is not optimal and hinders its widely clinical use. The rising of bone tissue engineering has provided a promising way to solve this problem, and has become one of the hotspots nowadays. However, the key technology of vascularization hasn't been solved ideally up to now. Obviously, the routine train of thoughts is faced up with big challenge in repaiering large bone defect. In order to find a clinically applicable bone tissue engineering approach, we brought forward and set up a microcirculation system of osteogenisis induction by perfusion in vivo.