Abstract: Objective Investigate the effect of the change of TrKA gene on the expression of neucleoprotein NF-κBp65 and apoptosis. Method To construct the expression vector of TrKA small interfering RNA,The recombinant was transfected into MCF-7 cells. the stable cell line expressing TrKA small interfering RNA were selected by G418. The mRNA and protein of TrKA were tested by real-time PCR,Western-blot and Immunohistochemistry.The change of neucleoprotein NF-κBp65 was detected by WB, Flow cytometry was used to observe the cell apoptosis. Result The expression vector of TrKA-siRNA was successfully constructed.The mRNA and protein of TrKA was decreased by 74.7% and 80. 5% respectively ( P < 0. 01).The positive control of GAPDH was decreased by 85.0% (p<0.05). The change of neucleoprotein NF-κBp65 was dramatical after treat the MCF-7 cells with NGF for 2 hours.but not to the siRNA cells. Compared with MCF-7 group,the apoptosis of the group of TrKA-siRNA increase obviously(p<0.05),but NGF can not enchance the effect. Conclusion Interfering the expression of TrKA gene effectively,could inhibit the activity of neucleoprotein NF-κBp65,increase the apoptosis of breast cancer cell MCF-7 independent of NGF.