Abstract: Objective To study the differences of trabecular meshwork cells cultured by perfused anterior segment and tissue sheet culture method, and to clarify their application in glaucoma study. Methods Trabecular meshwork cells were cultured by perfused anterior segment and tissue sheet culture method, light microscope was used to observe the trabecular meshwork cells and intercellular spaces. Results IOP of the porcine anterior segments perfused under constant rate at 0.1ml/h could maintain in the normal range（10~12mmHg）. The success rate was 50%. The IOP could be elevated with the increasing of perfusion rate, while the shape and structure of the organ were well preserved. Trabecular meshwork cultured by tissue sheet culture method could also reserve the tissues well, but the intercelluar spaces collapsed . Conclusion Perfused anterior segment could better simulate the normal physical state in situ and adequate perfusion was necessary for normal trabecular meshwork.