Abstract: Objective To understand the frequency of the 6 Polymorphism sites in the CETP gene in the healthy population of Hainan Li and Han nationality: TaqIB、I405V、D442G、R451Q、A373P and I14A .Method We detected the mutations of the 6 Polymorphism sites in the CETP gene in the healthy population of Hainan Li and Han nationality by the special allele specific-PCR system and analyzed the data by the statistics method. Result 3 genotypes can be detected according to TaqIB Polymorphism site in both Hainan Han and Li humanity which is B1B1，B1B2 and B2B2.3 genotypes can also be detected according to I405V Polymorphism site in both Hainan Han and Li humanity which is II,IV and VV. 2 genotypes can be detected according to D442G Polymorphism site in both Hainan Han and Li humanity which is DD and DG .Mutation of R451Q、A373P and I14A3 can't be detected both in Han and Li humanity. Genotype frequency（P<0.0001） and allele frequency（P<0.0001） of polymorphism site I405V exist significant difference between Hainan Han and Li humanity，while the other polymorphism sites exist no significant difference. Conclusion The frequent mutation sites of CETP gene in Hainan Han and Li humanity is TaqIB、I405V and D442G, of which I405V exist significant differenc, while the mutations in R451Q、A373P and I14A are infrequent.

Key words: cholesterol ester transfer protein (CETP) gene, DNA polymorphism, polymerase chain reaction (PCR), Li nationality, Han nationality