Abstract: Objective To study the histocompatibility of canine vascular smooth muscular-like cells(VSMLCs) and polyglycolic acid (PGA) coated with cross-linked collagen(PGA\collagen) scaffold by morphology observation ,cell proliferation and so on. Methods Rat's tail collagen and PGA\collagen scaffold were produced and constructed firstly. VSMLCs derived from bone marrow mesenchymal stem cells were cultured on the PGA\collagen scaffold. Morphological characteristics were observed by HE staining, toluidine blue staining and scanning electron microscope. Cells adhesion and proliferation were detected with MTT assay. Results The PGA\collagen scaffold had elasticity and tenacity. Histological and ultrastructural observation showed that there were lots of micropores in the scaffold. HE staining, toluidine blue staining ,scanning electron microscope and growth curve from MTT assay showed that VSMLCs seeded on the PGA\collagen scaffold grew well. Conclusion The PGA\collagen scaffold had a good histocompatibility with VSMLCs.

Key words: vacular smooth muscular-like(VSMLCs), vessel scaffold, polyglycolic acid(PGA), collagen, compatibility/cytocompatibility