Abstract: Objective Human colon cancer cells (lovo and RKO) were chose to study the anti-tumor effects of SFPS. Methods Inhibition of the cell proliferation was measured by MTT assay. SFPS inducing-apoptosis of lovo and RKO cells was observed by electron microscopy and flow cytometry. Results The potential of SFPS in inhibiting lovo and RKO cells viability was assessed by MTT assay. SFPS exhibited significantly antiproliferative activity which depended on dosage. Morphological examination showed chromosomal condensation, karyotheca margination, cell shrinkage and the presence of apoptosis bodies by electron microscopy. The overall effect of SFPS on the cell cycle distribution was examined by using flow cytometry. However, we also found that SFPS could arrest the human colon cancer cell line RKO at G0/G1 phase, and the RKO cells at S phase decreased significantly, while no changes of cell cycle distribution in SFPS treated lovo cells were observed. Conclusions SFPS may induce the apoptosis of lovo and RKO cells in vitro through anti-tumor proliferation.