Abstract: Objective To investigate the effect of gene gun transduction of Kras Antisense gene on the expression of Kras p21 protein in human pancreatic carcinoma cells.Methods Kras Antisense gene was transduced into pancreatic carcinoma cells by gene gun transduction, the expression of Kras p21 protein on BxPC-3、AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells line were observed by western blot and immunocytochemistry staining. Result The expression of Kras p21 protein in AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells was lower obviously after transduction of mutation specific Kras Antisense gene. Conclusion Gene gun transduction of Kras Antisense gene can significantly down-regulate the expression of Kras p21 protein in AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells, which can be an effective gene trerapy method in treating pancreatic carcinoma.

Key words: pancreatic carcinoma, gene gun, gene therapy, antisense gene