Abstract: Objective To test the antiproliferative and proapoptotic effect of SURVIVIN small interfering RNA (siRNA) expression vector on lung adenocarcinoma cell A549 in vitro. Methods The SURVIVIN-siRNA expression vector was constructed and conformed by sequencing. The inhibitory effect of SURVIVIN-siRNA was tested by fluorescent quantitative reverse transcription polymerase chain reaction (FQ RT-PCR)，Western blot and immunohistochemistry. The cell proliferation and apoptotic rate were assayed by tetrazolium bromide (MTT)colorimetry and flow cyclometry. Results SURVIVIN -siRNA expression vector was constructed and transfected into A549 cell successfully. It couldeffectively reduce the mRNA and protein level of SURVIVIN (76.4% and 84.5% respectively).