Abstract: Granulocyte colony-stimulating factor(G-CSF) is specific hematopoietic regulating growth factor of granulocyte lineage.It can be used to treat different kinds of granulocytopenia . Recently a variety of basic and clinical researches demonstrated that G-CSF can mobilize bone marrow stem cell and haemopoietic stem cell in the peripheral blood, which give us a new idea of releasing、enriching、mobolizing、promoting migration and inducing cell differentiation in the stem cell transplantation. The recent application of G-CSF in stem cell transplantation is summarized in this view.