Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (1): 33-37.

• 研究论文 • Previous Articles     Next Articles

Differentiation of mouse embryonic stem cells into cardiomyogenic cells cocultured with cardiomyocytes

Wei-dong LI, Xiao-gang ZHANG, Jing CHANG, Fang-pin JIANG   

  1. Department of Cardiology, the First Affiliated Hospital of Chongqing Medical University Department of Cardiology, the First Affiliated Hospital of Chongqing Medical University
  • Received:2007-12-13 Revised:2008-05-20 Online:2009-01-25 Published:2009-01-25
  • Contact: Xiao-gang ZHANG,

Abstract: Objective To explore the effect of embryonic stem cells(ESCs) differentiating into cardiomyocytes(CM ) by cardiomyocytes. Methods The blastocytes of 3.5d from Kunming species mouse,cultured on mouse embryonic fibroblaste cell feeder layers for 4~5d, and the mouse ESCs were isolated and subsequently cultured in vitro. Day 2~3 embryoid bodies(EBs) were derived from ESCs of passage 3 to 5 and then cocultured with neonatal cardiomyocyte(CM) to induce into cardiomyocytes and the expression of cardiac specific cardiac troponin-T(TnT) and а-Actin were detected by immunofluoresence. Result The rhythmic beating embryoid bodies were observed on day 3and reached 93% of rhythmic beating embryoid bodies on 12 day in group 2.All the beating cardiomyocytes derived from ESCs expressed cardiac-specific proteins for TnT and а-Actin, and the group induced by direct cell to cell contact acquired the highest differentiating ratio of 56.5%,and was the highest differentiating ratio compared with other groups (p<0.05).Conclusion Both cardiomyocyte coculturing system and cardiomyocyte-lysate system can induce mouse ESCs to differentiate into cardiomyocyte-like cells and the induced effect of direct cell-to-cell interaction with neonatal cardiomyocytes was higher than cardiomyocyte-lysate.

Key words: Embryonic stem cell, Coculture, Differentiation, Cardiomyocyte

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