Abstract: Objective To investigate three plant of vaginal Lactobacillus the regulative effects on macrophage and antitumor effects on murine uterocervical carcinoma number 14(U14) .Methods Established model mice transplanted with U14, then divided them into 5 groups at random.Model group and DDP group respectively were regarded as nagative and positive comparison ; Lactobacillus groups were injected 1010 each with Lactobacillus into peritoneum for 10 days successively. The TNF-α and NO content which the peritoneal macrophages secreted by KM mice secreted and the inhibitory rate on U14 cells were detected. Results The production of TNF-α and NO secreted by the peritoneal macrophages activated by Lactobacillus had greatly increased than Model control .The three strains of Lactobacillus all had the antitumor effects on KM mice bearing U14 significantly. Conclusion Lactobacillus showed a marked inhibition effects on the U14 cells and can activate macrophages to produce TNF-αand NO which may be related with the antitumor mechanism.